中国农业科技导报2024，Vol.26Issue(6)：214-225,12.DOI:10.13304/j.nykjdb.2023.0098
刀豆凝集素快速检测技术的研究现状及发展趋势
Research Status and Development Trend of Rapid Detection of Agglutinin in Concanavalin A
摘要
Abstract
Concanavalin A(Con A)is mainly found in jack bean,and it is a plant lectin that can specifically bind to polysaccharides,glycoproteins and other substances.Con A has anti-nutritional and anti-pest effects,which is an important part of plant self-protection,and also the main cause of food poisoning and allergic reactions caused by eating legumes.Therefore,the detection of Con A is of great significance for legume vegetable breeding and food safety assessment.The research progress of novel rapid detection technologies for Con A was introduced in detail,including nanomaterial based biosensor,immune sensing technology,sugar sensing technology and aptamer sensing technology.Biosensors based on nanomaterials had high accuracy but lack biological specificity;the immune sensing technology had improved the detection speed of the traditional immune method but still had the problem of insufficient signal repeatability;sugar sensing technology had greatly improved the specificity and detection accuracy of hemagglutination method,but it could not distinguish lectins with the same sugar specificity.Aptamers based detection technology had excellent detection accuracy,specificity and repeatability,and unnatural aptamers could be obtained through chemical modification to further enhance the specificity,which should be the development trend of rapid detection of Con A in the future.关键词
刀豆凝集素/食用安全性/快速检测技术/生物传感器/豆类蔬菜Key words
concanavalin A/edible safety/rapid detection/biosensor/legumes分类
农业科技引用本文复制引用
张笑笑,李晓倩,朱诚,吕晨泽..刀豆凝集素快速检测技术的研究现状及发展趋势[J].中国农业科技导报,2024,26(6):214-225,12.基金项目
国家自然科学基金青年基金项目(32201254) （32201254）
浙江省自然科学基金探索项目(LTGN23C020003). （LTGN23C020003）
