中国乳业Issue(5)：123-129,136,8.DOI:10.12377/1671-4393.24.05.23
牛羊乳脂肪球膜稳定性及非靶向脂质组学差异研究
Stability of Bovine and Goat Milk Fat Globule Membranes and Differences in Untargeted Lipidomics
摘要
Abstract
Chinese Holstein cows and dairy goats in Guanzhong region of China were used as research subjects to analyze milk fat globule membrane lipids based on non-targeted lipidomics technology to screen for membrane differential lipids.The results showed that 3 004 and 2 857 lipids were detected in cow and goat milk MFGM,respectively.Phospholipids,glycolipids,sterols and sphingolipids accounted for 75.94%,8.29%,3.82%and 3.76%of the total content of lipids other than glycerides in cow's milk as compared to 66.98%,12.42%,4.27%,and 3.57%in goat's milk,respectively.In addition,1 496 significantly differentiated lipids were present on bovine and sheep MFGM,in which glyceride molecules such as TG(17︰0/10︰0/22︰6),TG(16︰0e/8︰0/18︰1),TG(8︰0/10︰4/14︰2),TG(14︰0/14︰0/22︰6),and DG(15︰0/16︰0)were associated with the same lipid profile as CL(87︰12),PE(41︰2e),SM(t39︰7),CL(66︰1),MePC(29︰2e),MFGM lipid molecules such as PE(16︰0/16︰1)and PI(18︰1/20︰3)can be used for categorization and identification of cow's and sheep's milk as well as differentiation of the targets of the assay,which can provide the theoretical basis of the detection of cow's and sheep's milk lipids.关键词
牛羊乳/脂肪球膜脂质/脂质组学/差异分析Key words
breast's and goat's milk/lipid globule membrane lipid/fatty acid/lipidomics/variance analysis引用本文复制引用
孟毅,周银焜,魏怡,薛海燕,刘晓凤..牛羊乳脂肪球膜稳定性及非靶向脂质组学差异研究[J].中国乳业,2024,(5):123-129,136,8.基金项目
陕西省秦创原"科学家+工程师"队伍(2022KXJ-011),陕西科技大学博士启动基金(2023BJ-20),陕西省科技计划项目(2022GY-361,2020ZDLNY02-09) （2022KXJ-011）
