中国实验动物学报2024，Vol.32Issue(4)：461-467,7.DOI:10.3969/j.issn.1005-4847.2024.04.006
棕榈酸诱导斑马鱼脂毒性损伤骨形成抑制模型的建立
Establishment of a lipotoxic inhibition model of bone formation in zebrafish induced by palmitic acid
摘要
Abstract
Objective To establish a palmitic acid(PA)-induced model of lipotoxic bone formation inhibition in zebrafish.Methods AB strain zebrafish embryos were divided randomly into a blank control group,PA group,and simvastatin(SIM)group.Embryos in the PA and SIM groups received PA from 3 days post-fertilization(dpf),and embryos in the SIM group received SIM continuously for 4 days from 5 dpf.Establishment of the model was confirmed at 9 dpf by calcein staining,Nile red staining,triglyceride and total cholesterol content determination,and q-PCR.Results PA significantly decreased the number of vertebrae,promoted lipid accumulation,increased triglyceride and total cholesterol contents,promoted the expression of lipid-related genes PPARγ、c/EBPα,and inhibited the expression of osteogenic genes ALP and RUNX2.SIM improved the inhibitory effect of PA on bone formation in zebrafish.Conclusions PA can successfully create a lipotoxic model of bone-formation inhibition,similar to the pathological process of osteoporosis,using a simple,sensitive,and controllable method.This model can then be used for drug screening for osteoporosis and related diseases.关键词
骨质疏松模型/脂毒性/斑马鱼/棕榈酸/辛伐他汀Key words
osteoporosis model/lipotoxicity/zebrafish/palmitic acid/simvastatin分类
生物科学引用本文复制引用
王晓仪,李苗,王琳霞,喻斌,华永庆..棕榈酸诱导斑马鱼脂毒性损伤骨形成抑制模型的建立[J].中国实验动物学报,2024,32(4):461-467,7.基金项目
国家自然科学基金(81473390),江苏省高等学校自然科学研究重大项目(20KJA360001). Funded by National Nature Science Foundation of China(81473390),the Natural Science Foundation of the Jiangsu Higher Education Institutions of China(20KJA360001). （81473390）
