中国实验动物学报2024，Vol.32Issue(4)：468-476,9.DOI:10.3969/j.issn.1005-4847.2024.04.007
实验用大林姬鼠种群微卫星标记开发及遗传特性分析
Development of microsatellite markers and analysis of genetic characteristics of laboratory Apodemus peninsulae population
摘要
Abstract
Objective Polymorphic microsatellite markers developed for Apodemus peninsulae can enrich its genetic data and lay a foundation for genetic quality control and gene mapping.Methods Microsatellite loci were screened based on the genome sequence of Apodemus peninsulae,and microsatellite primers were identified.The genetic diversity of the population was analyzed by multiplex PCR.Results Thirty microsatellite markers were successfully developed and evaluated using 60 samples of Apodemus peninsulae.A total of 152 alleles were detected,with an average of 5.067 alleles per locus.The average observed heterozygosity was 0.592.The average Shannon index was 1.265.The average polymorphism information content was 0.598.Conclusions Based on the microsatellite loci developed in this study,the genetic diversity of Apodemus peninsulae can be effectively analyzed,laying a foundation for establishing genetic quality standards and detection method.关键词
大林姬鼠/微卫星位点/遗传多样性/封闭群Key words
Apodemus peninsulae/microsatellite loci/genetic diversity/closed colony分类
生物科学引用本文复制引用
张晴,张曦文,何松,袁宝,陈健,任文陟,权福实,胡进平,丁瑜..实验用大林姬鼠种群微卫星标记开发及遗传特性分析[J].中国实验动物学报,2024,32(4):468-476,9.基金项目
国家重点研发计划(2022YFF0710503),吉林省科技发展计划项目(20230505037ZP). Funded by the National Key Research and Development Program of China(2022YFF0710503),Jilin Provincial Science and Technology Development Plan Project(20230505037ZP). （2022YFF0710503）
