非酒精性脂肪性肝病大鼠的血清溶血磷脂酰胆碱和氨基酸代谢轮廓研究OA北大核心CSTPCD
Metabolic profiles of serum lysophosphatidylcholine and amino acids in rats with nonalcoholic fatty liver disease
目的 利用代谢组学手段研究非酒精性脂肪性肝病(nonalcoholic fatty liver disease,NAFLD)大鼠血清的氨基酸和溶血磷脂酰胆碱(lysophosphatidylcholine,LPC)代谢轮廓,寻找可表征NAFLD的生物标志物并推测其发生的可能机制.方法 通过喂养高脂饮食饲料和腹腔注射CCl4 制备NAFLD大鼠,采用液相色谱-质谱联用技术测定对照组和NAFLD组的大鼠血清中15 种LPCs和18 种氨基酸的含量;采用主成分分析和正交偏最小二乘法-判别分析等方法分析大鼠NAFLD肝损伤后血清中LPC和氨基酸代谢轮廓的变化,运用Pearson相关分析方法分析生物标志物与NAFLD的相关性.结果 NAFLD组的大鼠血清LPC和氨基酸代谢轮廓明显偏离对照组,且能完全区分开;LPC(20∶1)、精氨酸和谷氨酸对NAFLD有显著贡献,这 3 个代谢物被鉴定为生物标志物;LPC(20∶1)和精氨酸与AST、ALT、LDL、TBIL 等血清生化指标显著相关.结论 血清溶血磷脂酰胆碱和氨基酸的代谢轮廓与NAFLD密切相关.
Objective To use metabolomics method to study the metabolic profiles of amino acids and lysophosphatidylcholine(LPC)in the serum of rats with nonalcoholic fatty liver disease(NAFLD),to identify biomarkers for NAFLD,and to speculate on the possible mechanism responsible for its occurrence.Methods NAFLD rats were prepared by feeding a high-fat diet and intraperitoneal injection of carbon tetrachloride.Levels of 15 LPCs and 18 amino acids in the serum were determined in control and NAFLD rats by liquid chromatography-mass spectrometry.Changes in serum LPC and amino acid metabolic profiles in NAFLD rats were analyzed by principal component analysis and orthogonal partial least squares discriminant analysis.Correlations between biomarkers and NAFLD were analyzed by Pearson's correlation analysis.Results The metabolic profiles of serum LPC and amino acids differed significantly between the NAFLD group and the control group and were completely distinct.LPC(20∶1),arginine,and glutamic acid had significant contributions to NAFLD and were identified as biomarkers.Furthermore,LPC(20∶1)and arginine were significantly correlated with serum biochemical indicators such as aspartate transaminase,alanine transaminase,low-density lipoprotein,and total bilirubin.Conclusions The metabolic profiles of serum LPC and amino acids may be closely related to NALFD.
赵梅玉;史欣悦;周姝伶;李海军;樊淑婷;熊印华
江西科技师范大学药学院,南昌 330013
生物学
非酒精性脂肪性肝病溶血磷脂酰胆碱氨基酸代谢轮廓
nonalcoholic fatty liver diseaselysophosphatidylcholineamino acidsmetabolic profile
《中国实验动物学报》 2024 (004)
477-484 / 8
江西省教育厅科学技术研究项目(GJJ2201313),江西省高等学校精细化学品工程技术研究中心开放课题(JFCEC-KF-2202),大学生创新创业训练计划项目(20231704202),研究生创新专项资金项目(YC2023-S910). Funded by the Science and Technology Research Project of Jiangxi Provincial Department of Education(GJJ2201313),Open Project of Fine Chemical Engineering Technology Research Center of Jiangxi University(JFCEC-KF-2202),Innovation and Entrepreneurship Training Program for College Students(20231704202),Graduate Student Innovation Special Fund Project(YC2023-S910).
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