血友病性关节炎动物模型的研究进展
Progress in animal models of hemophilic arthritis
摘要
Abstract
Hemophilic arthritis(HA),caused by recurrent bleeding,can seriously affect patient quality of life and consumes extensive social and medical resources.There is thus a need to establish an animal model of HA for research;however,this is limited by ethical requirements.Here we review the recent literature and summarize research progress into animal models of HA at home and abroad,from the aspects of species selection,modeling method,histopathology,and imaging evaluation method.Species selection includes rodents such as mice,New Zealand rabbits,beagles,miniature pigs,and crab-eating macaques.Modeling method comprise gene knockout trauma models,gene knockout spontaneous models,and injection models.Among these,the gene knockout spontaneous model closely mimics the pathological process of spontaneous bleeding and concurrent arthritis in human HA,making it more relevant to human HA.However,due to high modeling costs,phenotypic instability,and low survival rates,this model is not the preferred choice for animal experimental studies.In contrast,gene knockout trauma models exhibit characteristics such as short modeling time,strong stability,and high success rates,thus being widely utilized in animal experimental research.Evaluation of HA models involves various imaging method including MRI,micro-CT,MSKUS/PD,in addition to various gross scoring method.By reviewing the progress of HA model research,more experimental evidence is provided for investigating the pathogenesis and validating the efficacy of HA treatments,thereby compensating for the lack of clinical data,particularly in the field of traditional Chinese medicine therapy.关键词
血友病性关节炎/动物模型/评价方法/组织病理学/影像学Key words
hemophilic arthritis/animal model/evaluation methods/histopathology/radiology分类
生物科学引用本文复制引用
林锟,于天启,张大光,何伟,魏秋实,何宪顺,田佳庆,陆舜,何敏聪,林天烨,何晓铭,范帅,杨宏光..血友病性关节炎动物模型的研究进展[J].中国实验动物学报,2024,32(4):523-529,7.基金项目
国家自然科学基金面上项目(82274544,81873327),国家自然科学基金青年科学基金(82004392),2022年度"揭榜挂帅"项目(毕科合重大专项[2022]1号),"双一流"与高水平大学学科协同创新团队重大项目(2021XK05),"双一流"与高水平大学学科协同创新团队培育项目(2021XK41),开放课题重点项目(GYH202101-01,GYH202101-04),国家中医优势专科建设项目(440000220000000043009). Funded by National Natural Science Foundation of China of General Project(82274544,81873327),National Natural Science Foundation of China of Youth Science Fund(82004392),"Unveiling Leadership"Project of 2022(Major Special Project[2022]No.1),Major Project of Synergistic Innovation of"Double First-Class"and High-Level University Disciplines(2021XK05),Incubation Project of Synergistic Innovation Team of"Double First-Class"and High-Level University Disciplines(2021XK41),Key Project of Open Topic(GYH202101-01,GYH202101-04),National Traditional Chinese Medicine Specialty Construction Project(440000220000000043009). （82274544,81873327）
