中国畜牧杂志2024，Vol.60Issue(6)：113-120,8.DOI:10.19556/j.0258-7033.20230211-05
猪卵巢circ_mmp16的鉴定及功能初探
A Preliminary Study on the Identification and Function of Circ_mmp16 in Porcine Ovaries
摘要
Abstract
The proliferative capacity of porcine follicular granulosa cells is closely related to reproductive traits.The aim of this study was to characterize circ_mmp16 and its regulatory network in porcine ovarian tissues,and to analyze its potential regulatory functions.The miRNAs and target genes targeted for regulation by circ_mmp16 were bioinformatically analyzed using miRanda and RNA hybird.The results showed that circ_mmp16 is a cyclic RNA present in mammals and conserved among species and consists of exons 2～4 of Matrix Metallopeptidase 16(Mmp16).In addition,circ_mmp16 can act as a competitive endogenous RNA for 26 miRNAs such as ssc-miR-217,ssc-miR-124a,and ssc-miR-16 to regulate downstream target genes.GO and KEGG analysis of the target genes of the circ_mmp16-miRNA regulatory network revealed that the Wnt signaling pathway,calcium transport,glutathione metabolism,and arachidonic acid metabolism were associated with ovarian function.Tissue expression profiling revealed that circ_mmp16 was most highly expressed in skeletal muscle,and second only to skeletal muscle in the ovary.At the level of porcine ovarian granulosa cells,circ_mmp16 was found to have a role in promoting the expression of cell cycle marker genes and to promote the expression of GPX3 and GPX4 glutathione synthesis marker genes.In conclusion,circ_mmp16,as a competitive endogenous RNA regulating multiple miRNAs,may play a role in protecting ovarian granulosa cells by promoting their proliferation as well as their resistance to oxidative stress,providing basic data to further reveal the involvement of granulosa cells in the regulation of follicular development and atresia in pigs.关键词
卵巢颗粒细胞/环状RNA/生物信息学分析/细胞增殖Key words
ovarian granulosa cells/cyclic RNA/bioinformatics analysis/cell proliferation分类
农业科技引用本文复制引用
张锫文,朱砺,潘宇恒,肖茜,顾洋,林许韬,邹冉,谢淑艳,沈林園,张辉..猪卵巢circ_mmp16的鉴定及功能初探[J].中国畜牧杂志,2024,60(6):113-120,8.基金项目
国家重点研发计划"农业生物种质资源挖掘与创新利用"重点专项资助(2021YFD1200801) （2021YFD1200801）
四川省科技计划(2021ZDZX0008、2021YFYZ0007、2021YFYZ0030、scsztd-2022-08-09) （2021ZDZX0008、2021YFYZ0007、2021YFYZ0030、scsztd-2022-08-09）
国家现代农业产业技术体系(CARS-35) （CARS-35）
