中国医科大学学报2024，Vol.53Issue(5)：385-391,7.DOI:10.12007/j.issn.0258-4646.2024.05.001
基于生物信息学构建宫颈癌预后相关ceRNA网络
Bioinformatics-based construction of a cervical cancer prognosis-associated competing endogenous RNA network
摘要
Abstract
Objective To comprehensively analyze the circulatory RNA-long non-coding RNA-microRNA-messenger RNA(circRNA-lncRNA-miRNA-mRNA)network in cervical cancer and construct a prognostic model.Methods Differential and key genes were ana-lyzed using bioinformatics based on data from The Cancer Genome Atlas(TCGA)and Gene Expression Omnibus(GEO)databases.Prognostic mRNA models were constructed based on TCGA database using univariate,Least Absolute Shrinkage and Selection Operator(LASSO),and multivariate Cox regression analyses and validated using the GEO database.The R package and Cystoscape software were used to construct a nomogram model and competing endogenous(ceRNA)network of circRNA-lncRNA-miRNA-mRNA in cervical cancer.Results A prognostic model including five mRNAs was constructed using univariate,LASSO,and multivariate Cox regression analyses,which had area under the receiver operating characteristic curve AUC values of 0.71,0.71,and 0.70 at 1,2,and 3 years,respec-tively,indicating its sensitivity and specificity in cervical cancer prognosis.Predictive results were validated using the GSE44001 dataset.The C-index of the nomogram model for this prognostic model was 0.707.In this study,a ceRNA network comprising 39 circRNAs,27 lncRNAs,12 miRNAs,and five mRNAs was constructed.Conclusion The network constructed in this study can help comprehensively elucidate the mechanism of ceRNAs in cervical cancer,and the construction of prognostic and Nomogram models can predict patient prog-nosis.关键词
竞争性内源RNA/环状RNA/长链非编码RNA/宫颈癌Key words
ceRNA/circular RNA/long non-coding RNA/cervical cancer分类
医药卫生引用本文复制引用
任敬秦,周常慧,刘成清,娜孜拉·赛提尼亚孜,杨楠,李榕..基于生物信息学构建宫颈癌预后相关ceRNA网络[J].中国医科大学学报,2024,53(5):385-391,7.基金项目
国家自然科学基金(82060468) （82060468）
中亚高发病成因与防治国家重点实验室(SKL-HIDCA-2022-GJ2) （SKL-HIDCA-2022-GJ2）
新疆维吾尔自治区"十四五"高等学校特色学科-公共卫生与预防医学 （）
