中国医科大学学报2024，Vol.53Issue(5)：401-404,413,5.DOI:10.12007/j.issn.0258-4646.2024.05.003
硫酸羟氯喹治疗狼疮性肾炎过程中肾小球内蛋白质表达谱的变化
Changes in the protein expression profiles of glomeruli during the treatment of lupus nephritis with hydroxychloroquine sulfate
摘要
Abstract
Objective To screen the differentially expressed proteins in glomeruli during the treatment of lupus nephritis(LN)with hydroxychloroquine sulfate(HCQ).Methods Sixty female NZB/WF1 mice were used.At the age of 28 weeks,40 mice with proteinuria of 3+to 4+were divided into HCQ and control groups.After feeding for 36 weeks,the glomerular proteins were extracted by magnetic beads and analyzed by two-dimensional fluorescence difference gel electrophoresis(2D-DIGE)and matrix-assisted laser desorption/ioniza-tion time-of-flight mass spectrometry(MALDI-TOF-MS).The expression of the candidate proteins in the glomeruli of the LN mice was exa-mined by immunohistochemistry.Results A total of 31 differentially expressed proteins were identified between the two groups,including 24 upregulated and seven downregulated proteins.Conclusion The expression of proteins like RI and ENOA in the glomeruli of LN mice was significantly different during HCQ treatment.These proteins may be involved in the pathogenesis of LN and are therefore potential targets of HCQ in the treatment of LN.关键词
肾小球/狼疮性肾炎/蛋白质组学/双向电泳Key words
glomerulus/lupus nephritis/proteomics/two-dimensional electrophoresis分类
医药卫生引用本文复制引用
李艳秋,王海潮,郑晓杰,刘晓丹,刘雪,田淑艳,姚丽..硫酸羟氯喹治疗狼疮性肾炎过程中肾小球内蛋白质表达谱的变化[J].中国医科大学学报,2024,53(5):401-404,413,5.基金项目
国家自然科学基金(81273107) （81273107）
沈阳市科技计划(211-173-9-67) （211-173-9-67）
