中国药物评价2024，Vol.41Issue(2)：105-109,5.
柑橘类药材中辛弗林含量研究
Study on the Content of Synephrine in Citrus spp.
摘要
Abstract
Objective:To compare the content of synephrine in Citrus spp.(Pericarpium citri reticulatae,Pericarpium citri reticulatae viride,Aurantii fructus immaturus,etc.),and provide reference for the quality evaluation of related medicinal materials.Methods:HPLC method for determination of synephrine in Citrus spp.was established.Using Waters SPHERISORB SCX(4.6 mm ×250 mm,5 μm)with a mobile phase of methanol-0.2％phosphoric acid solution(adjusted pH to 3.8 with ammonia test solution)(35∶65).The detection wavelength was 223 nm,and the column temperature was 30 ℃.The content of synephrine in Citrus spp.and different cultivars of Peri-carpium citri reticulatae were compared.Results:The linear range of synephrine was 2.842 8-568.560 6 μg·mL-1(r=1.000 0).The average recovery was 102.92％(RSD=1.39％,n=9).The content of synephrine in Citrus spp.were Qingpi＞Zhishi＞Dahongpao＞Chazhigan＞Chengpi＞Ningmeng＞Gouju.Synephrine was not detected in Foshou,Xiangyuan,Huajuhong,Youpi and Jinju.Among the different cultivars of Pericarpium citri reticulatae,Dahongpao had the highest synephrine content,followed by Ponkan,and Buzhihuo and Chunxiang had the lowest synephrine content.Conclusion:There was a certain correlation between the distribution of synephrine in Citrus spp.and its plant classification.The content of synephrine in Sinocitrus Tseng and Aurantium Miller were higher,and the content of syne-phrine in immature peel was higher than that in mature peel.The content of synephrine were also different between cultivars of Pericarpium citri reticulatae.This study could provide a certain reference and basis for the quality evaluation and application of Citrus spp..关键词
辛弗林/柑橘类药材/不同栽培品种Key words
Synephrine/Citrus spp./Different cultivars分类
医药卫生引用本文复制引用
许莉,陈允斌,文永盛,杨小艳,罗霄,代琪,高鹏..柑橘类药材中辛弗林含量研究[J].中国药物评价,2024,41(2):105-109,5.基金项目
国家重点研发计划——中医药现代化专项(2023YFC3504100) （2023YFC3504100）
