Abstract
Objective To establish a high-performance liquid chromatography-diode array detector(HPLC-DAD)method for simultaneous determination of nine components in Qipi Pills.Methods The chromatographic column was Agilent Extend C18 column(250 mm×4.6 mm,5 µm),the mobile phase was methanol acetonitrile(1∶1,V/V)-0.08%phosphoric acid aqueous solution(gradient elution),the flow rate was 1.0 mL/min,the detection wavelength was set at 237 nm for liquiritin and ammonium glycyrrhizinate,283 nm for hesperidin,203 nm for ginsenoside Rg1 and ginsenoside Re,220 nm for atracylenolide Ⅲ,atracylenolide Ⅱ,atracylenolide Ⅰ,and pachymic acid,the column temperature was 30℃,and the injection volume was 10 µL.Result The linear ranges of liquiritin,ammonium glycyrrhizinate,hesperidin,ginsenoside Rg1,ginsenoside Re,atracylenolide Ⅲ,atracylenolide Ⅱ,atracylenolide Ⅰ and pachymic acid were 3.66-58.48 µg/mL,6.21-99.41 µg/mL,9.53-152.54 µg/mL,3.06-48.89 µg/mL,1.51-24.11 µg/mL,2.54-40.60 µg/mL,1.24-19.78 µg/mL,2.10-33.64 µg/mL,and 3.57-57.08 µg/mL(r≥0.999 5,n=6).The RSDs of precision,repeatability,and stability test results were all lower than 2.0%(n=6).The average recoveries of the above nine components were 100.34%,101.35%,101.18%,99.65%,98.73%,98.86%,100.86%,99.30%and 100.86%,with RSDs of 1.43%,1.52%,0.68%,1.43%,1.00%,1.19%,1.31%,0.75%,and 1.36%(n=6),respectively.The contents of the above nine components in samples were in the ranges of 0.526 4-1.100 7 mg/g,0.956 2-1.700 2 mg/g,1.125 2-1.876 2 mg/g,0.570 9-0.825 6 mg/g,0.101 4-0.502 6 mg/g,0.226 9-0.611 7 mg/g,0.099 4-0.370 5 mg/g,0.152 5-0.416 4 mg/g,and 0.395 2-0.755 6 mg/g(n=3),respectively.Conclusion The method is simple,has strong specificity,good stability,and accurate results,which can be used for the simultaneous determination of nine components in Qipi Pills.关键词
高效液相色谱法/二极管阵列检测器/启脾丸/含量测定Key words
HPLC/DAD/Qipi Pills/content determination分类
医药卫生
