广东海洋大学学报2024，Vol.44Issue(3)：17-24,8.DOI:10.3969/j.issn.1673-9159.2024.03.003
肉桂醇提物对大口黑鲈虹彩病毒的体外抗病毒作用
Antiviral Effect of Ethanol Extracts of Cinnamomum cassia on Largemouth Bass Ranavirus
摘要
Abstract
[Objective]To investigate the antiviral effect and mechanism of ethanol extracts of Cinnamomum cassia in Largemouth bass virus(LMBV)infection,and to provide reference for the development of anti-LMBV drugs.[Method]The safe working concentration of cinnamon ethanol extract in fathead minnow muscle cell lines(FHM)was determined by CCK-8 kit,crystal violet staining,real-time quantitative PCR(RT-qPCR),and viral titer(TCID50),so as to evaluate the antiviral effect of cinnamon alcohol extract against LMBV at the cellular level,and to analyze the mechanism of its anti-LMBV activity.[Result]Cinnamon alcohol extract were not toxic to FHM cells at a mass concentration of≤12.5 μg/mL.After co-incubation of FHM cells with LMBV and cinnamon alcohol extract at mass concentrations of 12.5,6.25,and 3.125 μg/mL,respectively,the cellular lesions were significantly reduced,the number of viable cells increased significantly after crystal violet staining,and the expression of MCP and ICP46 genes was significantly reduced(P<0.01),and the antiviral effect was dose-dependent.12.5 μg/mL cinnamon alcohol extract is connected to FHM cells after co-incubation with LMBV,and the relative expression of MCP and ICP46 genes and virus titer(TCID50)in the cell decreased significantly(P<0.01);after FHM cells and LMBV treated with cinnamon alcohol extract co-incubation,the relative expression of LMBV MCP and ICP46 genes in cells is significantly lower than that of the LMBV infection group not treated with cinnamon alcohol extract(P<0.01);after LMBV infects FHM cells,the relative expression of MCP and ICP46 genes in FHM cells connected to cinnamon alcohol extract decreased significantly(P<0.05);the relative expression of MCP and ICP46 genes in FHM cells treated with cinnamon alcohol extract for 8 h,3 h after LMBV infection with FHM cells,was significantly decreased(P<0.05).[Conclusion]Cinnamon alcohol extract can reduce the infectious power of LMBV,interfere with the adsorption,invasion and replication of LMBV infecting host cells,has good anti-LMBV effects,and can be used as one of the candidates for environmentally friendly and efficient anti-LMBV fishery drugs.关键词
大口黑鲈/虹彩病毒/肉桂/抗病毒作用/渔用药物Key words
Micropterus salmoides/iridoviridae/Cinnamomum cassia/antiviral effect/fishery drug分类
农业科技引用本文复制引用
廖文钰,李鹏飞,竺利波,刘明珠,余庆,玉洁莹,陈嘉,韦云依,黄吉利,黄琳..肉桂醇提物对大口黑鲈虹彩病毒的体外抗病毒作用[J].广东海洋大学学报,2024,44(3):17-24,8.基金项目
国家自然科学基金(32373175) （32373175）
广西自然科学基金(2023JJG130007,2022GXNSFBA035521,2022JJA130074) （2023JJG130007,2022GXNSFBA035521,2022JJA130074）
国家重点研发计划(2022YFD2401200),国家现代农业产业技术体系广西创新团队项目(nycytxgxcxtd-2021-08-02). （2022YFD2401200）
