中南民族大学学报（自然科学版）2024，Vol.43Issue(4)：447-455,9.DOI:10.20056/j.cnki.ZNMDZK.20240403
制备核壳型多功能纳米载体用于化疗-光热治疗乳腺癌
Fabrication of multifunctional core-shell structured nanocarriers for chemo-photothermal therapy of breast cancer
摘要
Abstract
Black titanium dioxide(BT)was first obtained by the hydrogenation and reduction of white titanium dioxide(TiO2),then a core-shell structured nanocomposite(BT@M)consisting of BT core and degradable mesoporous organosilica(MON)shell containing bisulfide bonds was prepared as redox-responsive drug delivery nanocarriers.Subsequently,chemotherapeutic drug doxorubicin hydrochloride(DOX·HCl)was loaded in the mesoporous BT@M,followed by introducing the pH-responsive polydopamine(PDA)coating to the surface as a"nanovalve",to fabricate a dual stimulus-responsive drug delivery nanosystem(BT@M/D-P)for tumor chemo-photothermal therapy.Transmission electron microscopy(TEM)images of BT@M nanoparticles exhibited a distinct core-shell structure with a size of about 100 nm.In a reductive environment containing 10 mmol·L-1 glutathione(GSH),BT@M nanoparticles underwent the redox-triggered decomposition of the outer mesoporous organosilica shell,due to the breakage of disulfide bonds in the organosilica framework.Furthermore,BT@M/D-P displayed pH/redox dual stimuli-responsive drug release in simulated tumor microenvironment and physiological environments.Under 808 nm laser illumination,BT@M exhibited excellent photothermal stability and high photothermal conversion efficiency up to 35.5%.Under specific-wavelength light irradiation,BT@M/D-P exerted significantly higher toxicity against tumor cells than normal cells,via effective combination of conventional chemotherapy and photothermal therapy which"heated up"tumor cells.Compared with chemotherapy or photothermal therapy alone,the nanocomplex-based drug delivery system displayed combined chemo-photothermal therapeutic effects for breast cancer cells.Meanwhile,BT@M/D-P nanoparticles did not induce hemolysis at the concentration range of 50-200 µg‧mL-1,suggesting good blood compatibility.关键词
黑色二氧化钛/可降解介孔有机硅/药物可控释放/化疗-光热联合治疗Key words
black titanium dioxide/degradable mesoporous organosilica/controlled drug release/chemo-photothermal combination therapy分类
通用工业技术引用本文复制引用
余金鑫,程崟家..制备核壳型多功能纳米载体用于化疗-光热治疗乳腺癌[J].中南民族大学学报（自然科学版）,2024,43(4):447-455,9.基金项目
国家自然科学基金资助项目(52173295) （52173295）
中央高校基本科研业务费专项资金资助项目(CZQ23036) （CZQ23036）
