半夏泻心汤含药血清对人结肠癌细胞系SW480上皮-间质转化及TGF-β1/Smads通路的影响OACSTPCD

Objective:To explore the mechanism of pinellia heart-draining decoction(PHDD)on epithelial-mesenchymal transition of human colon cancer cell lines SW480.Methods:Thirty SD rats were randomly divided into PHDD group and blank group,with 15 rats in each group.PHDD and distilled water(24.68 g·kg-1)were administered daily for 7 consecutive days.Abdominal aortic blood was centrifuged to prepare PHDD-containing serum and blank serum.Colon cancer cell lines SW480 were used as the research object for this in vitro study.The cells were divided into control group,different concentrations of blank serum groups,and drug-containing serum groups.CCK-8 method was used to detect the survival rate of SW480 cells to screen the optimal drug intervention concentration and time.Cell migration was detected by scratch assay.Real-time fluorescence quantitative PCR(RT-qPCR)and Western blotting(WB)were used to detect the mRNA and protein expression of epithelial-mesenchymal transition proteins[E-cadherin,Vimentin]and TGF-β1/Smads pathway[transforming growth factor-β1(TGF-β1),Smads family protein 3(Smad3),Smads family protein 7(Smad7)]in each group.Results:Compared with the control group and the blank serum containing 10%group,the serum containing 20%PHDD could significantly inhibit the proliferation of SW480 cells(P<0.05,P<0.01),weaken the cell migration ability(P<0.01),reduce the expression of Vimentin,TGF-β1,Smad3 mRNA and protein,and increase the expression of E-cadherin mRNA and protein(P<0.05 or P<0.01),and increase the expression of Smad7 mRNA(P<0.01).Conclusion:Pinellia heart-draining decoction can inhibit the epithelial-mesenchymal transition of colon cancer cell SW480,and its mechanism may be involved in inhibiting the activation of TGF-β1/Smads pathway.