基于TLR7-MyD88-IRAK4信号通路的解表清里方体内抗甲型流感病毒的实验研究OACSTPCD

Objective:To investigate the effect and mechanism of Jiebiao Qingli formula(JQF)on in vivo inhibiting the reproduction of H1N1 influenza virus strain.Methods:Using the random number method,112 SPF-grade mice were equally divided into a blank group,a model group,an oseltamivir phosphate group(0.037 5 g/kg),and JQF high-,medium-,and low-dose groups(6.05,3.02,and 1.51 g/kg,respectively),with 22 mice in each group.The blank group was not subjected to any intervention,and the remaining groups of mice were infected with influenza A virus[A/Brisbane/02/2018(H1N1),pdm09-like virus(H1N1)]by nasal drip method.Two hours after modeling,the blank group and model group were given normal saline by gavage,the other groups were given correspanding doses of medication,and each group was given intragastric administration for 7 days.The body weights and activity status of the mice were examined daily at regular intervals during the administration period.Samples were taken from the mice on day 3 and day 7 of the modeling,and the lung index and pathological changes of the lungs were detected.The viral nucleic acid load in the lung tissues was detected by qT-PCR,and Western blotting was used to detect the protein expression levels of TLR7,MyD88,and IRAK4 in the lung tissues of the mice on day 7 of the modeling period.Results:JQF could alleviate the systemic symptoms,reduce the lung index,delay the weight loss of the mice(P<0.05),improve the pathological damage of lung tissues,reduce the viral titer in the murine lungs(P<0.05),and up-regulate the protein expression of TLR7,MyD88 and IRAK4 in lung tissues,with the best efficacy in the high-dose group of JQF.Conclusion:Jiebiao Qingli formula can dose-dependently improve lung infection and reduce viral replication in mice,and its mechanism may be related to the TLR7-MyD88-IRAK4 signaling pathway.