中医药学报2024，Vol.52Issue(6)：31-37,7.DOI:10.19664/j.cnki.1002-2392.240112
育阴灵加减方对D-半乳糖所致POI模型大鼠Hippo信号通路的影响
Effect of Modified Yuyinling Formula on Hippo Signaling Pathway in POI Model Rats Induced by D-galactose
摘要
Abstract
Objective:To study the effect of modified Yuyinling formula(YYLF)on POI model rats and its possible mechanism.Methods:The POI rat model was established by 35%D-galactose,40 female rats were randomly divided into model group,Chinese medicine high-dose(0.562 g/kg),medium-dose(0.281g/kg),and low-dose(0.141g/kg)groups,and Western medicine group.The Western medicine group was gavaged diethylstilbestrol solution at a dose of 0.120 mg/kg,and on the fourth day,mifepristone solution at a dose of 0.960 mg/kg was gavaged,and then,both drugs were discontinued for 1 day,as a treatment course,and four treatment courses were carried out.Eight non-modeled female rats were used as the blank group.Observations were made on the body weight of rats,ovarian and uterine coefficients,pathological changes in ovarian tissue,serum levels of anti-Müllerian hormone(AMH),inhibin B(INHB),and vascular endothelial growth factor(VEGF).The expression of Hippo signaling pathway-related effector factors in ovarian tissue were detected using protein immunoblotting and fluorescence quantitative PCR.Results:Compared with model group,body weight,ovarian coefficient and uterus coefficient of rats in high and medium dose group of YYLF were raised(P<0.05);YYLF could improve ovarian tissue structure,could raise the serum AMH,INHB and VEGF levels(P<0.05),and could reduce the expression levels of Hippo signaling pathway related effect factors Mst1,Lats2,YAP1,p-YAP and Smad7.Conclusion:Modified Yuyinling formula may improve the ovarian function of POI model rats by regulating the expression of Hippo signaling pathway.关键词
早发性卵巢功能不全/育阴灵加减方/卵巢结构/Hippo信号通路Key words
POI/Modified Yuyinling Formula/Ovary structure/Hippo signaling pathway分类
医药卫生引用本文复制引用
冯聪,齐娜,冯涛,李琛,张跃辉,王逸,白茸..育阴灵加减方对D-半乳糖所致POI模型大鼠Hippo信号通路的影响[J].中医药学报,2024,52(6):31-37,7.基金项目
黑龙江省自然科学基金联合引导项目(LH2022H079) （LH2022H079）
黑龙江省博士后特别资助项目(LBH-TZ2217) （LBH-TZ2217）
黑龙江省博士后资助面上项目(LBH-Z21079) （LBH-Z21079）
2023黑龙江省中医药科研项目(ZHY2023-084) （ZHY2023-084）
