类叶牡丹3种皂苷元对L929细胞炎症模型中HDAC表达的影响OACSTPCD

Objective:To investigate the inhibitory effects of three sapogenins from Cauorlhyllum Robustum Maxim on HDAC3/8 and their anti-rheumatoid arthritis(RA)activity.Methods:A TNF-α-induced inflammation model in L929 fibroblast cells was established in mice.The optimal drug concentration of the screened components and the optimal modeling concentration of TNF-α were determined using the CCK-8 method.The inflammation model was verified by ELISA using IL-6 concentration as an indicator,and the anti-inflammatory ability of the screened components was evaluated.Changes in HDAC3 and HDAC8 protein expression in L929 cells were detected using Western blot.Results:The optimal concentration of TNF-α was determined to be 10 ng/mL by the CCK-8 method.Hederagenin was used at concentrations of 500 μmol/L and 250 μmol/L,oleanolic acid at concentrations of 50 μmol/L and 25 μmol/L,and Echinocystic acid at concentrations of 25 μmol/L and 12.5 μmol/L.The IL-6 content in the L929 cell model group induced by TNF-α was significantly increased(P<0.01),and the different concentrations of drug groups showed different inhibitory effects on IL-6(P<0.05,P<0.01).The three sapogenins components significantly reduced the protein expression levels of HDAC3 and HDAC8 in the TNF-α-induced L929 cell inflammation model(P<0.05,P<0.01).Conclusion:In the TNF-α-induced L929 cell inflammation model,the three sapogenins from Cauorlhyllum Robustum Maxim showed good anti-inflammatory effects by reducing HDAC overexpression.