国际神经病学神经外科学杂志2024，Vol.51Issue(2)：29-34,6.DOI:10.16636/j.cnki.jinn.1673-2642.2024.02.006
G蛋白偶联受体30在大鼠蛛网膜下腔出血后对神经炎症和血脑屏障破坏的影响
GPR30 attenuates neuroinflammation and blood-brain barrier disruption in rats with subarachnoid hemorrhage
摘要
Abstract
Objective To investigate the effect of G protein-coupled receptor 30(GPR30)on neuroinflammation and blood-brain barrier(BBB)disruption during early brain injury(EBI)in rats with subarachnoid hemorrhage(SAH).Methods Thirty-six male rats were randomly divided into six groups(n=6 per group):sham operation(Sham)group and SAH(3 h,6 h,12 h,24 h,72 h)groups.In addition,72 rats were randomly divided into four groups(n=18 per group):Sham group,SAH group,SAH combined with overexpressed GPR30 lentivirus negative vector(SAH+Lv-NC)group,SAH combined with overexpressed GPR30 lentivirus vector(SAH+Lv-GPR30)group.A Sprague-Dawley rat model of SAH was established by intravascular perforation.Intraventricular injection of Lv-GPR30 was performed in the rats with SHA.The effect of GPR30 on EBI was analyzed through neurological scoring,brain tissue water content measurement,Evans blue staining,and HE staining.The levels of proteins and their transcription levels were determined by Western blotting and real-time fluorescence quantitative PCR,respectively.ELISA was employed to measure the levels of tumor necrosis factor-alpha(TNF-α),interleukin-6(IL-6),interleukin-1 beta(IL-1β),and interleukin-10(IL-10).Results Intraventricular injection of Lv-GPR30 increased the expression of GPR30 in the brain tissue of rats with SAH and improved their neurological function,neuroinflammation,BBB disruption,and cerebral edema.Overexpression of GPR30 inhibited the expression of matrix metallopeptidase-9 and matrix metallopeptidase-2 as well as the inflammatory factors TNF-α,IL-6,and IL-1β in the brain tissue of SAH rats but increased the IL-10 level.Conclusions GPR30 can alleviate neuroinflammation and BBB disruption in rats with SAH.关键词
蛛网膜下出血/G蛋白偶联受体30/早期脑损伤/血脑屏障/神经炎症Key words
subarachnoid hemorrhage/GPR30/early brain injury/blood-brain barrier/neuroinflammation分类
医药卫生引用本文复制引用
李智勇,陈政纲,彭俊..G蛋白偶联受体30在大鼠蛛网膜下腔出血后对神经炎症和血脑屏障破坏的影响[J].国际神经病学神经外科学杂志,2024,51(2):29-34,6.基金项目
海南省自然科学基金青年项目(821QN422). （821QN422）
