环境工程学报2024,Vol.18Issue(4):1183-1191,9.DOI:10.12030/j.cjee.202312127
有机碳源对异养硝化-好氧反硝化生物脱氮的影响及其优化
Effect of organic carbon on the biological nitrogen removal through heterotrophic nitrification-aerobic denitrification and its optimization
摘要
Abstract
Heterotrophic nitrification-aerobic denitrification(HN-AD)is a novel biological nitrogen removal technology for high-ammonium organic wastewater.Currently,related studies mainly focus on the HN-AD in pure culture using single bacteria,while studies on mixed bacteria systems suitable for realistic application scenario are relatively scarce,limiting the application of this technology.This study developed and validated an activated sludge domestication method with HN-AD function.Subsequently,the impacts of carbon source types and C/N ratio were investigated and optimized.Results showed that the HN-AD sludge was successfully domesticated,with a low proportion of autotrophic nitrifying bacteria(<1%).Under the optimal condition of nitrogen removal,Thauera was gradually enriched and became the dominant functional bacterium in the HN-AD system,accounting for a high relative abundance of 69.7%.Organic carbon sources had a significant impact on the HN-AD nitrogen removal system.At a C/N ratio of 20 and the mixed carbon sources(sodium citrate,sodium acetate,sodium succinate,the related COD ratio of these carbon sources was 1∶1∶1)in the HN-AD system,the removal efficiency of ammonium and total nitrogen could reach 95%and 89.6%,respectively,and COD removal efficiency could reach 92%,which could achieve effective total nitrogen and organic matter removal.关键词
异养硝化-好氧反硝化/碳氮比/氨转化率/微生物群落/反应动力学Key words
heterotrophic nitrification-aerobic denitrification/C/N/ammonia conversion efficiency/microbial communities/reaction kinetics分类
资源环境引用本文复制引用
谭丰佚,刘新颖,党岩,李继云,徐康宁..有机碳源对异养硝化-好氧反硝化生物脱氮的影响及其优化[J].环境工程学报,2024,18(4):1183-1191,9.基金项目
国家自然科学青年基金资助项目(52300075) (52300075)