解放军医学院学报2024，Vol.45Issue(3)：283-289,7.DOI:10.12435/j.issn.2095-5227.2023.154
母代孕期应激在损伤成年子代认知功能中对CX3CL1/CX3CR1轴的影响
Effects of maternal stress during pregnancy on CX3CL1/CX3CR1 axis in impaired cognitive function of adult offspring
摘要
Abstract
Background Prenatal stress may impair the connection between central nervous system during offspring development,and the CX3CL1/CX3CR1 axis establishes a unique communication system between neurons and microglia.Objective To clarify whether prenatal stress has an impact on the CX3CL1/CX3CR1 axis in impairing offspring cognitive function and explore its mechanism of action.Methods Twelve pregnant SD rats were randomly divided into prenatal stress group and control group,with 6 rats in each group.The prenatal stress group was subjected to restrictive stress on the 15th to 21st day of pregnancy,while the control group was fed normally.ELISA was used to detect plasma glucocorticoids in maternal and offspring peripheral blood,and cognitive function in adult offspring was evaluated using four behavioral methods:open field,elevated cross maze,new object recognition,and Y-maze.Immunofluorescence staining and Western blot were used to detect the expression of CX3CL1,CX3CR1,and Iba-1.Results The prenatal stress group and its offspring had elevated levels of glucocorticoids(P＜0.05).In the open field experiment,the offspring of the prenatal stress group showed a significant decrease in the central area retention time compared to the control group(P＜0.05),and a significant decrease in the number and retention time of entering the open arm in the elevated cross maze compared to the control group(P＜0.05),indicating anxiety like behavior.In the recognition of new objects,the recognition index significantly decreased(P＜0.05),indicating a decrease in learning and memory abilities.In the Y-maze,the recognition of new arms was significantly reduced(P＜0.05),indicating a decrease in spatial memory abilities,and increased expression of Iba-1(P＜0.05),CX3CR1(P＜0.05),and CX3CL1(P＜0.05)activated by prenatal stress in offspring microglia during pregnancy.Conclusion Prenatal stress damage to cognitive function in adult offspring may be related to CX3CL1/CX3CR1 communication disorders.关键词
孕期应激/糖皮质激素/小胶质细胞/CX3CL1/CX3CR1/认知功能障碍Key words
prenatal stress/glucocorticoids/microglia/CX3CL1/CX3CR1/cognitive dysfunction分类
医药卫生引用本文复制引用
柴冰茹,白生宾,田晓寒,吕梦姣,张馨怡,徐枭喻,张黎敏,李政,谷雨,廖礼彬..母代孕期应激在损伤成年子代认知功能中对CX3CL1/CX3CR1轴的影响[J].解放军医学院学报,2024,45(3):283-289,7.基金项目
国家自然科学基金项目(82160218) （82160218）
新疆医科大学大学生创新训练项目(202210760021) （202210760021）
