农业机械学报2024，Vol.55Issue(5)：431-439,9.DOI:10.6041/j.issn.1000-1298.2024.05.041
不同酶解条件下大豆分离蛋白结构特性及起泡性研究
Effect of Enzymatic Hydrolysis on Structure and Foamability of Soybean Protein Isolate
摘要
Abstract
Soybean protein isolate(SPI)was used as raw material for hydrolysis with Alcalase(0～180 min).The structural changes of enzymatic hydrolysis products were investigated by gel electrophoresis,Fourier transform infrared spectroscopy(FT-IR)and intrinsic fluorescence spectra.The interfacial behavior of enzymatic hydrolysis products was described by surface tension and adsorption of interfacial protein,and the influence of structural changes and interfacial behavior on the foam properties was analyzed.After enzymatic hydrolysis,the typical 7S and 11S bands disappeared and new bands were produced(about 24 ku).Compared with SPI,the content of a-helix was decreased,the content of β-turn and random coil was increased in hydrolysate,and the fluorescence wavelength was red shifted.These results indicated that the protein structure was unfolded,which in turn promoted the change of protein function.The results showed that the foamability of the sample was the best(143.20％)at 90 min,which may be due to the lowest average particle size(208.10 nm),high solubility(90.44％)and lowest surface tension of the hydrolysate at this time,which was conducive to improving the adsorption rate of the hydrolysate at the air-water interface.However,due to the small peptides produced by enzymolysis,the ability of protein network structure was lost,which had a negative impact on foam stability.In addition,the antioxidant activity of the protein was greatly improved by enzymatic hydrolysis.The foamability of SPI can be effectively improved through enzymatic hydrolysis,and the application range of SPI as an effective foaming agent in food was expanded.关键词
大豆分离蛋白/碱性蛋白酶/界面行为/起泡性/抗氧化Key words
soybean protein isolate/alcalase/interfacial behavior/foamability/antioxidant分类
轻工纺织引用本文复制引用
齐宝坤,王英,李子玉,张小影,王帅..不同酶解条件下大豆分离蛋白结构特性及起泡性研究[J].农业机械学报,2024,55(5):431-439,9.基金项目
国家重点研发计划项目(2021YFD2100301) （2021YFD2100301）
