水生态学杂志2024，Vol.45Issue(3)：165-171,7.DOI:10.15928/j.1674-3075.202203010054
增温对黑斑侧褶蛙蝌蚪形态表型和生理特征的影响
Effects of Increased Temperature on Morphological Phenotypes and Physiological Traits of Pelophylax nigromaculatus Tadpoles
摘要
Abstract
In this study,we explored the effects of increasing temperature on the morphological pheno-type and physiological traits on the tadpoles of dark-spotted frogs,Pelophylax nigromaculatus.Three temperature treatments(26℃,28℃,and 30℃)were set for the study,with each treatment run in tripli-cate.The metamorphosis duration from Gosner stage 37 tadpoles to Gosner stage 46 froglets(complete metamorphosis)was recorded,and the morphological phenotypes and physiological traits of Gosner stage 46 froglets were determined,including the sizes of body and internal organs,percentage of different leu-kocyte subtypes in the blood,ratio of neutrophils to lymphocytes(N/L),and responsiveness to phytohe-magglutinin(PHA-P).One-way ANOVA and multiple comparisons were used to analyze differences among the temperature treatment groups.Results show:(1)The increased temperature shortened the meta-morphosis duration from Gosner stage 37 tadpoles to stage 46 froglets,but no significant differences were found among the temperature groups(P＞0.05).The body size of froglets,as well as the kidney wet mass index decreased significantly with increased temperatures(P＜0.05).No significant differences were found among different temperature treatments for any other organ wet mass index or the length index of different digestive tract segments(P＞0.05).(2)With increased temperature,both the percentage of neutro-phils and N/L decreased while the percentage of basophils significantly increased(P＜0.05).(3)The maximal value for the PHAP-P response of froglets in the 26℃,28℃,and 30℃ treatments appeared at 1 h,2 h,and 2 h after injection,and the differences were not significant(P＞0.05).Increased temperatures shortened the body size of froglets and reduced kidneys wet mass at complete metamorphosis stage,weakened stress response capacity and immune function,and a tradeoff occurred between the number of neutrophils and basophils.We therefore conclude that climate warming is detrimental to the survival of froglets.关键词
黑斑侧褶蛙/水温/形态表型/变态时长/生理特征/应激反应Key words
Pelophylax nigromaculatus/water temperature/morphological phenotype/metamor-phosis duration/physiological traits/stress response分类
农业科技引用本文复制引用
裴鑫怡,王萍,彭秀娟,刁迎珠,李文慧,伍亮,张志强..增温对黑斑侧褶蛙蝌蚪形态表型和生理特征的影响[J].水生态学杂志,2024,45(3):165-171,7.基金项目
安徽农业大学2020年度省级重点教学研究项目(2020jyxm0531)和2021年度省级线下课程项目(2021xxkc039) （2020jyxm0531）
安徽农业大学2021年校级(XJDC202110364053)和省级大学生创新创业训练项目(S202110364019) （XJDC202110364053）
安徽农业大学2023年度校级动物学教学创新团队项目(2023aujxtd004). （2023aujxtd004）
