水生态学杂志2024，Vol.45Issue(3)：172-177,6.DOI:10.15928/j.1674-3075.202203040058
铜鱼华双身虫的宿主特异性及其寄生鳃的组织病理学
Host Specificity of Sindiplozoon coreius and Gill Histopathology of Coreius guichenoti After Infection
摘要
Abstract
Coreius guichenoti is a rare fish endemic to the upper reaches of Yangtze River.However,a diplozoid parasite has been detected in the gills of C.guichenoti during artificial breeding,seriously af-fecting breeding and release.In this study,we clarified the pathogenic mechanism of diplozoid parasitism in C.guichenoti cultured in a breeding base,and the diplozoids were identified as Sindiplozoon coreius.Histopathological sections of C.guichenoti gills infected by SS.coreius were stained with hematoxylin-eo-sin and the results show that S.coreius infection caused inflammatory reactions,such as hyperplasia,adhe-sion and eosinophilia of gill lamellae,as well as gill extrusion and deformation.In addition,diplozoids were found on gills of Schizothorax prenanti and Percocypris pingi from the same rearing system as C.guichenoti,and Procypris mera from the Xijiang River system in Guangxi Zhuang Autonomous Region,and all were identified as S.coreius based on morphological and molecular identification.The body size of diplozoids parasitizing the four fish species differed,but the morphological characteristics were identical.When ITS2 was used as marker,sequence similarity of Sindiplozoon parasitizing S.Prenanti,P.pingi and P.mera was over 99％with that parasitizing C.guichenoti,and the sequence similarity was over 96％with another Sindiplozoon species(Sindiplozoon ctenopharyngodoni).When COI was used as marker,the similarity of diplozoids among the four host fish species was 100％.Kimura 2-parameter dis-tance between diplozoids parasitizing the four hosts(0.29-0.84％)were much smaller than those between them and S.ctenopharyngodoni(3.52-4.33％),and all clustered at the base of the phylogenetic tree.Our results suggest that the host specificity of S.coreius is low,and species in the same family or genus should not be cultured in the same rearing system to avoid co-infection.关键词
单殖吸虫/铜鱼华双身虫/组织病理学/宿主特异性Key words
Monogenea/Sindiplozoon coreius/histopathological sections/host specificity分类
生物科学引用本文复制引用
曹诗宜,杨波,廖小林,朱滨,邹红,李明,吴山功,王桂堂,李文祥..铜鱼华双身虫的宿主特异性及其寄生鳃的组织病理学[J].水生态学杂志,2024,45(3):172-177,6.基金项目
财政部和农业农村部国家大宗淡水鱼产业技术体系(CARS-45) （CARS-45）
国家自然科学基金(32373179). （32373179）
