生态学杂志2024，Vol.43Issue(5)：1234-1242,9.DOI:10.13292/j.1000-4890.202405.038
根系-菌根-土壤微生物对毛竹林土壤氮矿化过程的贡献
Contribution of roots-mycorrhizae-free-living microorganisms to soil nitrogen mineralization in moso bam-boo forest
摘要
Abstract
As a key process of soil nitrogen(N)cycle,soil N mineralization drives N supply capacity of soil.How-ever,the contribution of different biological factors such as roots,mycorrhizal mycelia,and asymbiotic microorgan-isms to soil N mineralization is poorly understood.In this study,we examined soil N mineralization of moso bamboo forest(Phyllostachys edulis)in Miaoshanwu Nature Reserve in Zhejiang Province,employing in situ incubation technique with anionic and cationic resin cores.Combined with different mesh sizes(1.45 mm:allowing all compo-nents to enter;53 μm:excluding roots but allowing mycelium to enter;and 1 μm:excluding roots and mycelium to enter while only allowing free-living microorganisms to enter),the microcosm further distinguished the effects of roots,mycelium and free-living microorganisms.The results showed that:(1)In the treatments of different mesh size microcosms,the exclusion effect of 53 µm microcosm on roots reached 100％compared with 1.45 mm micro-cosm,and the exclusion effect of 1 μm microcosm on hyphae reached 70％compared with 53 μm microcosm.(2)The participation of different biological components in the soil N cycling mainly changed soil ammonification rate.Soil net ammonification rate after root system participated in the soil N cycle process(1.45 mm microcosm)was 36.58％lower than that when the roots were excluded(53 μm microcosm).However,there was no significant difference in soil net nitrification rate and net mineralization rate among microcosm devices with different mesh sizes.(3)The soil leucine aminopeptidase(LAP)and phenoloxidase(POX)activities were significantly higher in the 1.45 mm microcosm compared to the 53 μm microcosm by 27.59％and 61.54％,respectively,which in turn led to a decrease in soil net ammonification rate.This study quantified the contribution of rhizosphere(root,mycor-rhizal mycelia)-mediated soil N mineralization process of bamboo forest.Free-living microorganisms are important biological factors affecting soil net nitrification and mineralization,while roots and mycorrhizal fungi played an important role in soil net ammonification.关键词
竹林/土壤氮循环/根际/菌根真菌/净硝化速率/净氨化速率/净矿化速率Key words
bamboo forest/soil nitrogen cycling/rhizosphere/mycorrhizal fungi/net nitrification rate/net ammo-nification rate/net mineralization rate引用本文复制引用
包涛涛,李丝雨,王一,蒋文婷,蔡延江,孙连鹏,栾军伟..根系-菌根-土壤微生物对毛竹林土壤氮矿化过程的贡献[J].生态学杂志,2024,43(5):1234-1242,9.基金项目
国家自然基金面上项目(31971461)、十四五重点研发计划课题(2021YFD2200405)和(2021YFD2200403)和中央公益性科研院所基本科研业务费学科发展项目(1632019006和1632021023)资助. （31971461）
