生物骨科材料与临床研究2024，Vol.21Issue(3)：8-14,7.DOI:10.3969/j.issn.1672-5972.2024.03.002
纳米多孔β-磷酸三钙复合自体骨折血肿的异位成骨潜能的初步实验研究
A preliminary experimental study on the ectopic osteogenesis potential of nano-porous β-tricalcium phosphate composite autologous fracture hematoma
刘豪 1杨佩 2王春生 2张旭 1刘琦 1刘芳 1熊执政1
作者信息
- 1. 湖南师范大学附属岳阳医院骨科,湖南 岳阳,414000
- 2. 西安交通大学第二附属医院骨科,陕西 西安,710006
- 折叠
摘要
Abstract
Objective To investigate the ectopic osteogenesis potential of nano-porous β-tricalcium phosphate complex autogenous fracture hematoma.Methods Seventy-eight New Zealand white rabbits were randomly divided into 5 groups:Group A,B,C,D and E.Bone fragments of approximately 5 mm×10 mm×10 mm were extracted from the upper part of the iliac bone to create an artificial fracture and form hematoma,then hematoma/β-TCP,β-TCP,hematoma/iliac crest and iliac crest were implanted under latissimus dorsal muscle,and sublatissimus dorsal muscle specimens were removed at 1,4 and 8 week after the establishment of the model.New bone formation,degradation of scaffold materials and biomechanical tests were evaluated by pathological staining and immunohistochemistry.Group E was used to observe the hematoma while observing the adsorption inside the β-TCP stent.The healthy iliac bone of group E animals was set as a normal control(group F),and only biomechanical tests were performed.Results A large number of cells and extracellular matrix were filled in the scaffold on day 4 after iliac fracture,and the mechanical properties of hematoma/β-TCP were slightly better than that of β-TCP scaffold alone.There were more new bone tissue and vascular tissue around the specimens of group A and group C,while only a small amount of scattered osteoid formation could be seen in the specimens of group B and group D,and the absorption of specimens of group D was obvious.There was no significant difference in the residual scaffold volume between group A and group B(P>0.05).The new bone mass of group A and group C increased with the extension of time(P<0.01),but the change of new bone mass of group B and group D had no statistical significance with the extension of time(P>0.05).Conclusion β-TCP has good compatibility with the active ingredients in fracture hematoma,and β-TCP has definite osteogenic potential after fracture hematoma,which is expected to be a new treatment strategy for old fractures and bone defects.关键词
骨折血肿/β-磷酸三钙/骨再生/骨缺损/组织工程Key words
Fracture hematoma/β-tricalcium phosphate/Bone regeneration/Bone defect/Tissue engineering分类
医药卫生引用本文复制引用
刘豪,杨佩,王春生,张旭,刘琦,刘芳,熊执政..纳米多孔β-磷酸三钙复合自体骨折血肿的异位成骨潜能的初步实验研究[J].生物骨科材料与临床研究,2024,21(3):8-14,7.
