酿酒酵母液泡电导1的抗原表位预测及抗原编码基因的克隆OACSTPCD

For the preparation of the antigen protein of yeast vacuolar conductance 1(Yvc1),the bioinformatics methods were applied in the prediction for antigen epitope of Yvc1,and the corresponding antigen encoding gene was cloned in this study.The prediction for antigen epitopes of Yvc1 in Saccharomyces cerevisiae S288c was conducted by using NCBI GenBank database and various bioinformatics software,such as ORP Finder,EXPASY-ProtScale,SOPMA,IEDB,NetMHC Ⅱ pan,etc.The results showed that YVC1 gene contained 2 028 bp in length and 33 open reading frames,with the longest one encoding 675 amino acids.As a stable and hydrophilic protein with molecular weight of 7.793 7×104,Yvc1 was located in vacuole membrane with 4 extramembrane regions.Regarding the secondary structure,29.48％and 3.11％amino acids were involved in the formation of random coil and β-turn,respectively.Moreover,our prediction showed there were 5 potential dominant linear B cell epitopes and 2 potential dominant helper T cell epitopes in this protein,with the dominant antigen epitope in the amino acid sequence from 1 to 236.Notably,the gene sequence similarity reached 99％between the amplified antigen encoding gene in YVC1 of S.cerevisiae DL5168 and the predicted gene from 487 707-489 734 bp in the strain S288c.Overall,the antigen encoding gene was successfully cloned according to the predicted results by bioinformatics methods,providing a foundation for further preparation of Yvc1 antigen protein in S.cerevisiae.