圆红冬孢酵母利用废糖蜜合成微生物油脂及类胡萝卜素OACSTPCD

Rhodosporidium toruloides is an excellent microbial oil production strain due to its wide substrate spectrum,stable performance and high cell oil content.Moreover,it can be used to produce valuable carotenoids with high yield,which has high valuable potential.Herein,we successfully developed a new fermentation method for the conversion of low-cost carbon source molasses to highly valuable microbial oil and carotenoids using R.toruloides Z11.By using the disaccharide strategy of co-adding molasses and glucose for the conversion,this method could not only save cost,but also increase microbial oil and carotenoid production.Notably,this process was conducted without exogenous addition of organic nitrogen sources and limiting phosphate.Based on previous results that the two-stage regulation strategy of temperature and pH could improve the fermentation biomass,oil content and carotenoids production,we explored a variety of fermentation strategies.Compared with the fermentation using single glucose as carbon source,the disaccharide strategy with R.toruloides Z11 led to 2.72-fold increase for biomass(OD600,up to 379.0±0.3).Meanwhile,the oil content and the carotenoid level reached 68.9％and 2.58 mg/g,respectively.