核酸功能化纳米探针检测水体中痕量Pb2+的研究OACSTPCD

Lead ion(Pb2+),one of the three major heavy metal pollutants,can seriously harm the environment and human health.Herein,we developed a simple and economical method for the detection of Pb2+in water using AuNP-DNAzyme nanoprobes,which were constructed with gold nanoparticles(AuNP)as fluorophore labeled deoxyribonuclease(DNAzyme)carrier and quench agent.Through the specific recognition of Pb2+by DNAzyme and subsequent Pb2+-catalyzed fragmentation of substrate chain,the fluorescence signal could be restored for analytical purpose.As a result,our method showed high sensitivity for Pb2+,with linear detection at 5-125 nmol/L range and calibration limit of 3.41 nmol/L.In addition,our sensor demonstrated excellent anti-interference performance to Pb2+and proved to be effective for the analysis of Pb2+added river water samples,leading to 95.90％-100.83％recovery.Our research suggests the sensor with AuNP-DNAzyme nanoprobe can potentially have broad application in the field of environmental monitoring.