生物加工过程2024，Vol.22Issue(3)：302-308,7.DOI:10.3969/j.issn.1672-3678.2024.03.008
核酸功能化纳米探针检测水体中痕量Pb2+的研究
Nucleic acid functionalized nanoprobs for the detection of trace Pb2+in aqueous media
摘要
Abstract
Lead ion(Pb2+),one of the three major heavy metal pollutants,can seriously harm the environment and human health.Herein,we developed a simple and economical method for the detection of Pb2+in water using AuNP-DNAzyme nanoprobes,which were constructed with gold nanoparticles(AuNP)as fluorophore labeled deoxyribonuclease(DNAzyme)carrier and quench agent.Through the specific recognition of Pb2+by DNAzyme and subsequent Pb2+-catalyzed fragmentation of substrate chain,the fluorescence signal could be restored for analytical purpose.As a result,our method showed high sensitivity for Pb2+,with linear detection at 5-125 nmol/L range and calibration limit of 3.41 nmol/L.In addition,our sensor demonstrated excellent anti-interference performance to Pb2+and proved to be effective for the analysis of Pb2+added river water samples,leading to 95.90％-100.83％recovery.Our research suggests the sensor with AuNP-DNAzyme nanoprobe can potentially have broad application in the field of environmental monitoring.关键词
铅离子/DNAzyme/纳米金颗粒/AuNP-DNAzyme纳米探针/重金属污染Key words
lead ion/DNAzyme/gold nanoparticles/AuNP-DNAzyme nanoprobe/heavy metal pollution分类
化学引用本文复制引用
张勤,姜涵,李向玲,宋天顺,谢婧婧..核酸功能化纳米探针检测水体中痕量Pb2+的研究[J].生物加工过程,2024,22(3):302-308,7.基金项目
国家自然科学基金面上基金(22078149) （22078149）
江苏省高等学校自然科学面上项目(20KJB150020) （20KJB150020）
