实验科学与技术2024，Vol.22Issue(3)：47-53,7.DOI:10.12179/1672-4550.20230562
自噬流的诱导与检测综合性实验的设计与实践
Design and Practice of the Comprehensive Experiment of Induction and Detection of Autophagic Flux
摘要
Abstract
One of the important ways to improve the experimental teaching level and the talent training quality is dismantling,refining and adding the international frontier theories and technologies to the experimental teaching in time.The comprehensive experiment of induction and detection of autophagic flux is designed,which includes cell passage culture,cell transfection and induction and observation of autophagy.A systematic,comprehensive and exploratory modular experimental teaching content is formed.Through double fluorescent labeled autophagosome marker protein expressed in cells and starvation inducing,autophagosomes and autophagic flux are observed by the fluorescence microscope.The practice results in undergraduate experimental teaching indicate that it is helpful to cultivate students'scientific research thinking and the innovative spirit,and improve their ability to analyze and solve problems.关键词
自噬/自噬流/细胞生物学/综合实验Key words
autophagy/autophagic flux/cell biology/comprehensive experiment分类
生物学引用本文复制引用
杨娇艳,刘凯于,张金菊,王泽群,李兵,杨勇波..自噬流的诱导与检测综合性实验的设计与实践[J].实验科学与技术,2024,22(3):47-53,7.基金项目
华中师范大学中央高校基本科研业务费项目(科研成果转化实验教学内容类)(CCNU21KYZHSY11) （科研成果转化实验教学内容类）
湖北省高等学校省级教学研究项目(2022089) （2022089）
国家自然科学基金面上项目(61977028) （61977028）
湖北省自然科学基金(2022CFB322). （2022CFB322）
