中国水产科学2024，Vol.31Issue(3)：301-315,15.DOI:10.12264/JFSC2023-0248
武陵山西部及邻近地区宽鳍鱲群体遗传多样性和遗传结构
Population genetic diversity and genetic structure of Zacco platypus in the western Wuling Mountains and adjacent areas
摘要
Abstract
To assess the germplasm genetic diversity of Zacco platypus populations in the western Wuling Mountains and Shennongjia Forestry District,we analyzed the genetic diversity and structure of seven Z.platypus populations using mitochondrial Cyt b sequences and microsatellite DNA(SSR)markers.Our results revealed 144 variant sites and 33 haplotypes among 185 Cyt b sequences from the seven populations.The haplotype diversity(Hd)ranged from 0.000 to 0.887,and the nucleotide diversity(π)ranged from 0.000 to 0.275.The neighbor-joining(NJ)phylogenetic tree and median-joining network of Cyt b haplotypes grouped the seven populations into three branches:Jiangkou1/Jiangkou2(JK1/JK2),Shennongjia(SNJ),Xishui/Chishui/Wuchuan/Nanchuan(XS/CS/WC/NC).Analysis of molecular variance(AMOVA)indicated significant genetic differentiation among the seven populations and among the three branches.Analyses based on 11 microsatellite DNA loci showed that the mean number of alleles(NA)in the seven populations of Z.platypus was 3.66,the mean Shannon's information index(Ⅰ)was 0.689,the mean observed heterozygosity(Ho)was 0.315,the mean expected heterozygosity(HE)was 0.354,and the mean polymorphic information content(PIC)was 0.409.The genetic differentiation index(FST)among the populations varied from 0.060 to 0.547,with significant genetic differentiation among the populations(P＜0.05).The UPGMA phylogenetic tree and PCoA showed that the seven Z.platypus populations clustered into four clades.STRUCTURE clustering analysis revealed that the seven populations of Z.platypus could be classified into four genetic groups.AMOVA showed significant genetic differentiation among the seven populations and among the four genetic groups of Z.platypus.Hence,the wild Z.platypus populations in the western Wuling Mountains and Shennongjia Forestry District exhibit low genetic diversity and significant genetic differentiation.Strengthening the protection of the riverine ecology and environment is crucial to ensure the sustainable utilization of wild germplasm resources of Z.platypus.关键词
宽鳍鱲/Cytb序列/微卫星DNA/遗传多样性/遗传结构Key words
Zacco platypus/Cyt b sequences/microsatellite DNA/genetic diversity/genetic structure分类
农业科技引用本文复制引用
覃宁,张利铖,肖梦娜,周旭,张桂蓉,马徐发,魏开建..武陵山西部及邻近地区宽鳍鱲群体遗传多样性和遗传结构[J].中国水产科学,2024,31(3):301-315,15.基金项目
生态环境部生物多样性调查评估项目(2019HJ2096001006). （2019HJ2096001006）
