武陵山西部及邻近地区宽鳍鱲群体遗传多样性和遗传结构OA北大核心CSTPCD
Population genetic diversity and genetic structure of Zacco platypus in the western Wuling Mountains and adjacent areas
为评估武陵山西部和神农架林区宽鳍鱲(Zacco platypus)群体的种质遗传多样性,采用线粒体Cyt b序列和微卫星DNA(SSR)标记对7个宽鳍鱲群体的遗传多样性和遗传结构进行了分析.结果显示,7个群体185个Cyt b序列中共检测出144个变异位点和33个单倍型,群体间的单倍型多样性(Hd)为0.000～0.887,核苷酸多样性(π)为0.000～0.275.Cyt b单倍型的NJ系统树和中介网络图显示,7个群体聚为3个分支(江口 1/江口 2,神农架,习水/赤水/务川/南川).分子方差分析(AMOVA)显示7个群体之间、3个支系之间存在显著的遗传分化.基于11个微卫星DNA位点的分析表明,宽鳍鱲7个群体的平均等位基因数(NA)为3.66,平均Shannon's信息指数(I)为0.689,平均观测杂合度(Ho)为0.315,平均期望杂合度(HE)为0.354,平均多态信息含量(PIC)为0.409.群体之间的遗传分化指数(FST)变化范围为0.060～0.547,各群体之间存在显著遗传分化(P＜0.05).UPGMA系统树和PCoA分析显示,7个宽鳍鱲群体聚为4支,STRUCTURE聚类分析显示宽鳍鱲7个群体可划分为4个最佳遗传组群.AMOVA分析表明,宽鳍鱲7个群体之间、4个遗传组群之间存在显著的遗传分化.结论认为,武陵山西部及神农架林区的野生宽鳍鱲群体整体上具有较低的遗传多样性,且存在显著的遗传分化,应加强对河流生态和环境的保护,确保宽鳍鱲野生种质资源的可持续利用.
To assess the germplasm genetic diversity of Zacco platypus populations in the western Wuling Mountains and Shennongjia Forestry District,we analyzed the genetic diversity and structure of seven Z.platypus populations using mitochondrial Cyt b sequences and microsatellite DNA(SSR)markers.Our results revealed 144 variant sites and 33 haplotypes among 185 Cyt b sequences from the seven populations.The haplotype diversity(Hd)ranged from 0.000 to 0.887,and the nucleotide diversity(π)ranged from 0.000 to 0.275.The neighbor-joining(NJ)phylogenetic tree and median-joining network of Cyt b haplotypes grouped the seven populations into three branches:Jiangkou1/Jiangkou2(JK1/JK2),Shennongjia(SNJ),Xishui/Chishui/Wuchuan/Nanchuan(XS/CS/WC/NC).Analysis of molecular variance(AMOVA)indicated significant genetic differentiation among the seven populations and among the three branches.Analyses based on 11 microsatellite DNA loci showed that the mean number of alleles(NA)in the seven populations of Z.platypus was 3.66,the mean Shannon's information index(Ⅰ)was 0.689,the mean observed heterozygosity(Ho)was 0.315,the mean expected heterozygosity(HE)was 0.354,and the mean polymorphic information content(PIC)was 0.409.The genetic differentiation index(FST)among the populations varied from 0.060 to 0.547,with significant genetic differentiation among the populations(P＜0.05).The UPGMA phylogenetic tree and PCoA showed that the seven Z.platypus populations clustered into four clades.STRUCTURE clustering analysis revealed that the seven populations of Z.platypus could be classified into four genetic groups.AMOVA showed significant genetic differentiation among the seven populations and among the four genetic groups of Z.platypus.Hence,the wild Z.platypus populations in the western Wuling Mountains and Shennongjia Forestry District exhibit low genetic diversity and significant genetic differentiation.Strengthening the protection of the riverine ecology and environment is crucial to ensure the sustainable utilization of wild germplasm resources of Z.platypus.
覃宁;张利铖;肖梦娜;周旭;张桂蓉;马徐发;魏开建
华中农业大学水产学院,湖北武汉 430070||贵州省水产研究所,贵州贵阳 550025华中农业大学水产学院,湖北武汉 430070
水产学
宽鳍鱲Cytb序列微卫星DNA遗传多样性遗传结构
Zacco platypusCyt b sequencesmicrosatellite DNAgenetic diversitygenetic structure
《中国水产科学》 2024 (003)
301-315 / 15
生态环境部生物多样性调查评估项目(2019HJ2096001006).
评论