中国实验动物学报2024，Vol.32Issue(5)：566-575,10.DOI:10.3969/j.issn.1005-4847.2024.05.003
运动调节Nrf2/HO-1通路改善HFFC膳食诱导肝细胞氧化应激的作用研究
Effects of exercise regulated the Nrf2/HO-1 pathway on improving HFFC diet-induced oxidative stress in hepatocytes
摘要
Abstract
Objective To explore whether voluntary wheel running affects liver oxidative stress by regulating the Nrf2/HO-1 pathway,thereby alleviating HFFC diet-related lipid deposition in the liver.Methods Eight-week-old C57BL/6J mice were randomly divided into a normal diet group(NC group,n=10)and high-fat,fructose,and cholesterol diet group(HFFC group,n=20)after 1 week of adaptive feeding.Ten weeks of feeding later,mice in the HFFC group were divided into a quiet group(HFFC group,n=10)and HFFC combined with exercise group(HFFC+EX group,n=10).HFFC+EX group mice were caged with voluntary running wheels for free movement,and the number of running wheels was recorded every day for 8 weeks.After the last treatment,the mice were sacrificed by fasting for 12 hours at an interval of 24 hours,and the blood and liver were collected for analysis.Results(1)Body weight,liver weight,and liver index of mice fed the HFFC diet were significantly higher than those of the NC group,which significantly decreased after exercise(P＜0.05).(2)Compared with the NC group,HDL-C and LDL-C in the HFFC group were significantly increased,and the LDL-C level was significantly decreased after 8 weeks of exercise(P＜0.05).(3)The liver fat droplet area and liver TG content in the HFFC group were significantly higher than those in the NC group,whereas those in HFFC+EX group were significantly decreased(P＜0.05).(4)Compared with the NC group,the content of oxidase MDA in the HFFC group were significantly increased,and nuclear translocation and gene expression of Nrf2 were significantly decreased.After exercise,the activities of SOD and T-AOC were significantly increased,and the nuclear translocation and gene expression of Nrf2 and expression levels of HO-1 and SOD-1 were significantly increased(P＜0.05).(5)The number of apoptotic hepatocytes and CHOP expression in the HFFC diet group were significantly higher than those in the NC group,whereas the number of apoptotic hepatocytes,and CHOP and Bax/Bcl-2 expression in the exercise group were significantly lower than those in the NC group(P＜0.05).Conclusions Voluntary wheel can alleviate HFFC diet induced liver lipid deposition by regulating the Nrf2/HO-1 pathway,thereby alleviating oxidative stress and reducing apoptosis in liver cells.关键词
肝/自主跑轮运动/氧化应激/核因子E2相关因子2/血红素加氧酶-1Key words
liver/voluntary wheel running/oxidative stress/nuclear factor erythroid 2-related factor 2/heme oxygenase-1分类
生物科学引用本文复制引用
平烨,张珮雯,袁馨梦,项梦奇,杨梦凡,林小霞,董诗茹,刘宇婷,张媛..运动调节Nrf2/HO-1通路改善HFFC膳食诱导肝细胞氧化应激的作用研究[J].中国实验动物学报,2024,32(5):566-575,10.基金项目
国家自然科学基金青年项目(32000839),江苏省"青蓝工程"资助项目([2021]11),江苏省研究生科研与实践创新计划(KYCX22-2256,KYCX22-2247),江苏省大学生创新创业训练计划(202210330009Z).Funded by the Youth Progect of National Natural Science Foundation of China(32000839),"Qing Lan"Project of Jiangsu Province of China([2021]11),Graduate Research and Innovation Projects of Jiangsu Province(KYCX22-2256,KYCX22-2247),Jiangsu Province College Students'Innovation and Entrepreneurship Training Program(202210330009Z). （32000839）
