中国实验动物学报2024，Vol.32Issue(5)：576-584,9.DOI:10.3969/j.issn.1005-4847.2024.05.004
遗传多样性高血压小鼠模型的建立与基因转录调控分析
Establishment of a genetically diverse mouse model of hypertension and analysis of gene transcription regulation
摘要
Abstract
Objective To investigate the differences in blood pressure phenotypes,renal pathological changes,and related pathogenic pathways in genetically diverse hypertensive mice obtained from 13 strains.Methods The genotypes of Cckbr+/+,Cckbr+/-and Cckbr-/-were obtained by hybridization of 13 strains of genetically diverse mice with Cckbr-/-mice.Blood pressure was measured with a noninvasive blood pressure analysis system(BP-2000).The expression of CCKBR protein in mouse kidney tissue was detected by Western Blot,and the pathological changes in mouse kidney tissue were detected by hematoxylin-eosin(HE)staining and immunohistochemistry(IHC).The pathogenic pathways related to essential hypertension were screened by RNA sequencing.Results In three specific mouse strains(A/J,LOT,and FIM),the systolic blood pressure(SBP)was significantly different between the Cckbr-/-and Cckbr+/+groups.HE staining and IHC showed that hypertension caused a certain degree of renal injury in the mice.Gene Ontology(GO)and pathway enrichment analysis showed that differentially expressed genes were enriched in metabolic processes and circadian rhythm regulation.Conclusions Genetically diverse mice can effectively simulate the genetic background of the population and provide a new resource for studying the pathogenic genes related to essential hypertension.关键词
遗传多样性小鼠/Cckbr/原发性高血压/动物模型Key words
genetically diverse mouse/Cckbr/essential hypertension/animal model分类
生物科学引用本文复制引用
黄智滨,潘吉荣,张伶燕,赵大路,王谦,魏承志,马旭,白琳,秦川..遗传多样性高血压小鼠模型的建立与基因转录调控分析[J].中国实验动物学报,2024,32(5):576-584,9.基金项目
中国医学科学院医学与健康科技创新工程(2021-I2M-1-034),国家重点研发计划(2021YFF0703200).Funded by CAMS Initiative for Innovative Medicine of China(2021-I2M-1-034),National Key R&D Program of China(2021YFF0703200). （2021-I2M-1-034）
