中国实验动物学报2024，Vol.32Issue(5)：592-599,8.DOI:10.3969/j.issn.1005-4847.2024.05.006
小鼠血液中年龄相关的DNA甲基化微单倍型的筛选与验证
Screening and validation of age-related DNA methylation microhaplotypes in mouse blood
摘要
Abstract
Objective The DNA methylation microhaplotype(DMH)refers to the combination of multiple methylation sites within a very short range,and these haplotypes show wide diversity.We carried out screening and validation of age-related DMHs in mouse blood.Methods We initially constructed a theoretical dataset of DMHs based on the mouse reference genome.We then screened age-related DMHs by Spearman's rank correlation analysis,using high-throughput sequencing information for DNA methylation in mouse blood from a network database.Finally,cross-validation was performed using a validation dataset.Results A total of 6787 142 DMH sites were identified within 50 bp in the mouse genome,including 98.64%of single-digit CpG sites.A total of 5835 age-associated DMHs were screened in 58 mouse blood samples(|rho|＞0.5,P＜0.01),accounting for 0.086%of DMHs.Finally,we validated the top 100 age-associated DMHs with high correlation in 95 independent samples,Resultsing in 44 loci.Conclusions The age-associated DMHs screened in this study may be useful in future studies of apparent age prediction using mouse blood and in aging studies.关键词
血液/DNA甲基化微单倍型/Spearman秩相关系数/年龄/筛选Key words
blood/DNA methylation microhaplotype/Spearman's rank correlation coefficient/age/screening分类
生物科学引用本文复制引用
田艺博,吴雨静,肖君华,周宇荀,李凯..小鼠血液中年龄相关的DNA甲基化微单倍型的筛选与验证[J].中国实验动物学报,2024,32(5):592-599,8.基金项目
国家重点研发计划(2018YFA0801101).Funded by National Key Research and Development Program of China(2018YFA0801101). （2018YFA0801101）
