中国比较医学杂志2024，Vol.34Issue(4)：20-27,8.DOI:10.3969/j.issn.1671-7856.2024.04.003
黄花菜醇提物对斑马鱼抑郁样行为的改善作用研究
Improvement of depressive-like behavior in Hemerocallis citrina Baroni using a zebrafish model
摘要
Abstract
Objective To investigate the effects of alcoholic extract of Hemerocallis citrina Baroni on the depressive-like behaviors in zebrafish larvae(Danio rerio)induced by reserpine.Methods Zebrafish larvae were divided into various groups:control(Con)group,reserpine group,fluoxetine group,H.citrina alcohol extract(HCE)low dose group(1.5 mg/L),HCE medium dose group(3 mg/L),and HCE high dose group(4.5 mg/L).Depressive-like behaviors were analyzed using sound and light stimulation.Real-time PCR was used to investigate the effects of HCE on depression related astrocyte markers(GFAP,C3,C4B,EMP-1,S100α-10)and the neurotrophic factor BDNF and its receptor genes(P75,TrkB).Results In comparison to the control group,the model group demonstrated significantly shorter movement distance and reduced movement time under sound and light stimulation(P＜0.05,P＜0.001,P＜0.0001).Following the administration of HCE,zebrafish larvae exhibited significantly heightened sensitivity to light and sound stimulation compared to the model group(P＜0.05,P＜0.0001).Astrocyte marker genes were up-regulated in the model group zebrafish brains compared to the control group(P＜0.0001).However,when the model group was administered HCE,the expression of astrocyte markers was significantly down-regulated compared to the model group(P＜0.0001).Neurotrophic factor and its receptor genes(BDNF,P75,TrkB)were down-regulated in zebrafish brains in the model group compared to those in the control group(P＜0.0001).However,in the group administered HCE,the expression of BDNF,P75,and TrkB was significantly up-regulated compared to that in the model group(P＜0.01,P＜0.0001).These findings suggest that HCE suppressed the inflammatory responses caused by astrocyte activation and promoted the production of neurotrophic factors and their receptor genes,thereby exerting an ameliorative effect on depression.Conclusions Alcoholic extracts of H.citrina can ameliorate the depression-like behavioral changes induced by reserpine in zebrafish larvae.They reduce the expression of astrocyte markers in the zebrafish brain and promote the production of neurotrophic factors and their receptor genes,playing an antidepressant role.关键词
黄花菜/抑郁症/利血平/斑马鱼Key words
Hemerocallis citrina Baroni/depression/reserpine/zebrafish分类
医药卫生引用本文复制引用
郑涵文,王梓航,刘昕玥,于佳玮,Alberto Carlos Pires Dias,王凤忠,王琼..黄花菜醇提物对斑马鱼抑郁样行为的改善作用研究[J].中国比较医学杂志,2024,34(4):20-27,8.基金项目
航天医学基础与应用国家重点实验室开放基金资助项目(SMFA18K06) （SMFA18K06）
国家重点研发计划(2021YFD1600100) （2021YFD1600100）
高端外国专家引进计划(G2022051012L). （G2022051012L）
