中国比较医学杂志2024，Vol.34Issue(4)：28-34,7.DOI:10.3969/j.issn.1671-7856.2024.04.004
五行音乐对孕期恐应激子代社交行为的影响及机制研究
Effects and mechanism of five-element music on social behavior of offspring of stress-injured pregnant mice
摘要
Abstract
Objective To investigate the effects and mechanisms of five-element music on the social behavior of the children of mothers with fear stress during pregnancy and provide a basis for the early prevention and treatment of clinical fetogenic affective disorders.Methods Forty-five pregnant mice were randomly divided into three groups:a control group,model group,and five-element music group.The model and five-element music group models were established using the bystander electric shock method.Additionally,the five-element music group was exposed to Palace Tune five-element music daily from 17:00 to 19:00 during pregnancy.On the 19th day of pregnancy,ELISA was employed to assess the levels of adrenocorticotropin(ACTH)and cortisol(CORT)in the serum of pregnant mice in each group for modeling evaluation.The offspring were subsequently grouped with their mother and underwent an 8-week-old three-box social experiment to observe their social behavior.We used the immunofluorescence double-labeling method to detect glutamatergic neuron activity in the medial prefrontal cortex(mPFC)of the offspring.High-performance liquid chromatography was employed to measure the total glutamate(Glu)content in the mPFC,while Gorky staining was used to observe changes in the dendritic spines of mPFC neurons in the offspring.Results Compared to those in the blank group,pregnant mice in the model group exhibited a significant increase in the levels of ACTH and CORT in their serum,and there was a significant decrease in the social interaction time and social novelty preference index of their offspring.There was also a significant decrease in glutamate neuron activity,glutamate content,and neuronal dendritic spine density.In contrast,compared with those in the model group,pregnant mice in the five-element music group demonstrated a reduction in the levels of ACTH and CORT in the serum,and there were improvements in the social behavior,glutamate neuron activity,glutamate content,and condition of neuronal dendritic spines in the offspring.Conclusions Intervention with five-element music effectively ameliorated the offspring's social behavior disorder result ing from prenatal fear stress;the mechanism was potentially linked to enhanced glutamate neuron activity in the mPFC region.关键词
五行音乐/孕期恐应激/子代/社交行为/谷氨酸能神经元Key words
five-element music/fear stress during pregnancy/offspring/social interaction behavior/glutamatergic neurons分类
医药卫生引用本文复制引用
吴永叶,杨丽萍,张振强,赵佳佳,宋琦,侯俊林,王耀辉,李笑琳,关徐涛..五行音乐对孕期恐应激子代社交行为的影响及机制研究[J].中国比较医学杂志,2024,34(4):28-34,7.基金项目
国家自然科学基金(81973596) （81973596）
河南省大学生创新创业训练计划项目(202210471004). （202210471004）
