中国比较医学杂志2024，Vol.34Issue(4)：73-83,11.DOI:10.3969/j.issn.1671-7856.2024.04.009
山药多糖对地塞米松诱导IR-3T3-L1脂肪细胞的降血糖作用及机制研究
Hypoglycemic effect of Chinese yam polysaccharide on DEX-induced IR-3T3-L1 adipocytes
摘要
Abstract
Objective To examine the hypoglycemic effect of Chinese yam polysaccharide on the 3T3-L1 insulin resistance cell model.Methods IR-3T3-L1 adipocytes were randomly divided into control group(Con),model group(DEX),metformin group(Met,positive drug group)and CYPS group(0.05,0.15,0.45 mg/mL).After the creation of the IR-3T3-L1 cell model by dexamethasone(DEX)(1 μmol/L)stimulation,Chinese yam polysaccharide treatment was applied.Glucose intake and the levels of TC,TG,LDL-C,HDL-C,GSH-Px,MDA,HK,and PK were then measured.AMPK-PI3K/Akt signaling pathway-associated gene expression was identified using qRT-PCR.Results The glucose consumption of IR-3T3-L1 cells was considerably decreased after 48 hours of treatment with 1 μmol/L DEX compared to that of the Con group(P＜0.01),and the reduction persisted for 60 hours.0.05,0.15,0.45 mg/mL CYPS treatment significantly increased glucose consumption(P＜0.01),HK,PK,GSH-Px enzyme activities(P＜0.01),HDL-C content(P＜0.01),and decreased MDA enzyme activity(P＜0.01),T-CHO,TG,LDL-C content(P＜0.01)in IR-3T3-L1 cells.01).Additionally,CYPS administration dramatically decreased PI3K,Akt,GLUT-4,AMPK,IRS-2,PPARa,and adiponectin mRNA expression levels(P＜0.05)and reduced IRS-1,GSK-3β,ACC,and FAS mRNA expression levels(P＜0.01).Conclusions In IR-3T3-L1 cells,CYPS can reduce oxidative stress,control lipid metabolism disorders,and enhance DEX-induced glucose intake.AMPK-PI3K/Akt signaling pathway-associated genes may be connected to the process.关键词
山药多糖/胰岛素抵抗/地塞米松/3T3-L1细胞/二甲双胍/AMPK-PI3K/Akt信号通路Key words
Chinese yam polysaccharide/insulin resistance/dexamethasone/3T3-L1 cells/metformin/AMPK-PI3K/Akt signaling pathway分类
医药卫生引用本文复制引用
刘昕玥,王凤忠,郑涵文,Alberto Carlos Pires Dias,范蓓,王琼..山药多糖对地塞米松诱导IR-3T3-L1脂肪细胞的降血糖作用及机制研究[J].中国比较医学杂志,2024,34(4):73-83,11.基金项目
国家重点研发计划(2021YFD1600100) （2021YFD1600100）
高端外国专家引进计划(G2022051012L). （G2022051012L）
