中国药房2024，Vol.35Issue(11)：1315-1319,5.DOI:10.6039/j.issn.1001-0408.2024.11.06
含羞草根乙酸乙酯部位对急性髓系白血病小鼠的影响
Effect of ethyl acetate extract from Mimosa pudica root on acute myeloid leukemia in mice
摘要
Abstract
OBJECTIVE To study the inhibitory effect of ethyl acetate extract from Mimosa pudica root(ethyl acetate extract for short)on acute myeloid leukemia in mice.METHODS Different concentrations of ethyl acetate extract(0.062 5,0.125,0.25,0.5 mg/mL)were used to treat acute myelomonocytic leukemia cell lines WEHI-3,and their effects on cell viability were investigated.Fifty BALB/C mice were randomly divided into blank control group,model group,positive control group(5-fluorouracil,13 mg/kg),and ethyl acetate extract low-dose and high-dose groups(50,200 mg/kg),with 10 mice in each group.Except for the blank control group,the leukemia model was constructed by intraperitoneal injection of WEHI-3 cells in other groups,and from the second day of modeling,corresponding drugs/water were orally administered once a day for 14 consecutive days.After the last administration,the liver and spleen indexes of mice were measured,and liver tissue pathological morphology observation,hematological analysis,and white blood cell differentiation detection were performed;the levels of cytokine[interleukin-2(IL-2),IL-3,interferon-γ(IFN-γ),tumor necrosis factor-α(TNF-α)]in serum were determined;the levels of leukocyte surface markers[cluster of differentiation 3(CD3),CD19,CD11b,CD107b(Mac-3)]in whole blood were all detected.RESULTS After treated with 0.062 5-0.5 mg/mL ethyl acetate,the inhibition rate of cell proliferation were increased significantly(P＜0.05).After intervention with high-dose ethyl acetate,the liver and spleen index,serum level of TNF-α,the levels of CD11b and Mac-3 in blood were significantly reduced(P＜0.05),while serum levels of IL-2,IL-3 and IFN-γ,and the levels of CD3 and CD19 in blood were increased significantly(P＜0.05).Occasional lymphocyte infiltration was present in the liver parenchyma,with almost no infiltration of inflammatory cells;hematology improvement and weakened white blood cell differentiation were found.CONCLUSIONS The ethyl acetate extract of M.pudica root can inhibit the proliferation of WEHI-3 cells,and improve symptoms in acute myeloid leukemia mice,the mechanism of which may be associated with enhancing the immune function.关键词
含羞草根/乙酸乙酯部位/急性髓系白血病/白细胞分化/免疫功能Key words
Mimosa pudica root/ethyl acetate extract/acute myeloid leukemia/white blood cell differentiation/immune function分类
医药卫生引用本文复制引用
邓婉柔,李慧敏,曾元宁,王秋红..含羞草根乙酸乙酯部位对急性髓系白血病小鼠的影响[J].中国药房,2024,35(11):1315-1319,5.基金项目
国家重点研发计划项目(No.2018YFC1707100) （No.2018YFC1707100）
广东省重点领域研发计划项目(No.2020B111112-0002) （No.2020B111112-0002）
