中国药房2024，Vol.35Issue(11)：1327-1333,7.DOI:10.6039/j.issn.1001-0408.2024.11.08
桃红四物汤加味颗粒对糖尿病肾病模型大鼠足细胞上皮-间充质转化及肾脏纤维化的影响
Effects of Taohong siwu decoction modified granules on podocyte epithelial-mesenchymal-transition and renal fibrosis in rats with diabetic kidney disease
摘要
Abstract
OBJECTIVE To investigate the effects of Taohong siwu decoction modified granules on podocyte epithelial-mesenchymal-transition(EMT)and renal fibrosis in diabetic kidney disease(DKD)model rats.METHODS Eight rats were selected as normal group(ordinary feed);the remaining rats were given a high-glucose and high-fat diet combined with intraperitoneal injection of streptozotocin(35 mg/kg)to induce the DKD model.Model rats were randomly divided into model group,irbesartan group[positive control,13.5 mg/(kg·d)]and modified Taohong siwu decoction group[6.48 g/(kg·d)],with 8 rats in each group.All groups were given relevant medicine intragastrically,once a day,for 16 consecutive weeks.Twenty-four-hour urinary total protein(24 h UTP)was detected at the end of the 4th,8th,12th and 16th week of administration.After the last medication,the body mass,water intake,food intake,urine output,the levels of fasting blood glucose,serum creatinine(Scr)and blood urea nitrogen(BUN)as well as mRNA and protein expressions of P-cadherin,nephrin,α-smooth muscle actin(α-SMA),Wilms'tumor gene 1(WT1),transforming growth factor-β1(TGF-β1)and type Ⅳ collagen(Col-Ⅳ)in renal tissue were determined.The pathological and morphological changes in renal tissue were observed and the thickness of the glomerular basement membrane was determined.RESULTS Compared with the model group,24 h UTP of rats was significantly decreased in modified Taohong siwu decoction group since the 8th weekend(P＜0.05);the body weight of rats increased significantly,but the amount of water intake and urine decreased significantly;Scr and BUN level,mRNA expression of α-SMA,mRNA and protein expressions of TGF-β1 and Col-Ⅳ were significantly reduced,while the mRNA expressions of P-cadherin,nephrin and WT1 were increased significantly(P＜0.05);the protein deposition of α-SMA was reduced,protein depositions of P-cadherin,nephrin and WT1 were increased;the pathological damage and fibrosis of renal tissue were relieved;the thickness of glomerular basement membrane was decreased significantly(P＜0.05).CONCLUSIONS Taohong siwu decoction modified granules can inhibit the EMT of podocyte in DKD model rats,and alleviate renal pathological damage and podocyte damage,thus protecting renal function,and delaying the process of renal fibrosis.关键词
糖尿病肾病/桃红四物汤加味颗粒/肾脏纤维化/足细胞/上皮-间充质转化Key words
diabetic kidney disease/Taohong siwu decoction modified granules/renal fibrosis/podocyte/epithelial-mesenchymal-transition分类
医药卫生引用本文复制引用
白璐,沈士朋,吴苏,郭帅,刘茂东..桃红四物汤加味颗粒对糖尿病肾病模型大鼠足细胞上皮-间充质转化及肾脏纤维化的影响[J].中国药房,2024,35(11):1327-1333,7.基金项目
河北省中医药管理局中医药类科研计划项目(No.2019131,No.2022408) （No.2019131,No.2022408）
