中国药房2024，Vol.35Issue(11)：1334-1338,5.DOI:10.6039/j.issn.1001-0408.2024.11.09
薯蓣皂苷对脓毒症大鼠肾损伤的影响及机制
Effect and mechanism of dioscin on renal injury in septic rats
摘要
Abstract
OBJECTIVE To investigate the effect of dioscin on renal injury in septic rats and its possible mechanism.METHODS The septic rat model was induced by using cecal ligation and puncture.Sixty model rats were randomly divided into model group(0.5%sodium carboxymethyl cellulose solution),dioscin low-dose,medium-dose and high-dose groups(30,60,120 mg/kg)and dexamethasone group(positive control,10 mg/kg),with 12 rats per group;another 12 rats were selected as the sham operation group(0.5%sodium carboxymethyl cellulose solution).After 15 minutes of modeling,rats in each group were injected with medicine/0.5%sodium carboxymethyl cellulose solution via the tail vein.Twenty-four hours after administration,the levels of creatinine(Cr),blood urea nitrogen(BUN),neutrophil gelatinase associated lipocalin(NGAL),kidney injury molecule-1(KIM-1),interleukin 6(IL-6),IL-1β and tumor necrosis factor-α(TNF-α)in serum and malondialdehyde(MDA)in renal tissue,superoxide dismutase(SOD)activity and the protein expressions of nuclear factor E2-related factor 2(Nrf2),heme oxygenase-1(HO-1),NOD-like receptor protein 3(NLRP3)were detected;renal histomorphology was observed.RESULTS Compared with model group,pathological injury of renal tissue was improved significantly in dioscin low-dose,medium-dose and high-dose groups;the levels of Cr,BUN,NGAL,KIM-1,IL-6,IL-1β and TNF-α in serum,MDA level and protein expression of NLRP3 in renal tissue were decreased significantly(P＜0.05);SOD activity in renal tissue,protein expressions of Nrf2 and HO-1 were increased significantly(P＜0.05),in a dose-dependent manner(P＜0.05).The pathological damage of renal tissue in the dioscin high-dose group was similar to dexamethasone group,and there was no statistically significant difference in the levels of the above indicators(P＞0.05).CONCLUSIONS Dioscin can activate the Nrf2/HO-1 signaling pathway to inhibit NLRP3 inflammasome,and realize the inhibition of inflammatory factors and oxidative stress,so as to protect the kidney injury in sepsis.关键词
薯蓣皂苷/Nrf2/HO-1信号通路/NLRP3炎性小体/脓毒症/肾损伤Key words
dioscin/Nrf2/HO-1 signaling pathway/NOD-like receptor protein 3 inflammasome/sepsis/renal injury分类
医药卫生引用本文复制引用
申翔,徐尚刚,黄洋辉,罗斌,周裕丰,梁隆斌..薯蓣皂苷对脓毒症大鼠肾损伤的影响及机制[J].中国药房,2024,35(11):1334-1338,5.基金项目
四川省卫生健康委员会科研课题(No.21PJ032) （No.21PJ032）
