安徽农业科学2024，Vol.52Issue(12)：1-6,12,7.DOI:10.3969/j.issn.0517-6611.2024.12.001
根瘤菌对茎瘤芥的促生作用研究
Study on the Growth-promoting Effect of Rhizobia on Tumorous Stem Mustard
摘要
Abstract
[Objective]To study the infection of Azorhizobium caulinodans in tumorous stem mustard and its effect on plant growth promotion.[Method]The differences in growth promoting effects of rhizobia on different varieties of tumorous stem mustard,the effects of different concen-trations of rhizobia on the cultivated variety'Fuza 2'of tumorous stem mustard and the effects of different inducers on nodulation were ana-lyzed.[Result]The root length and plant height of'Fulza 2'increased significantly,and the growth promotion effect was obvious among the 6 stem mustard varieties infected with nitrogen-fixing rhizobia.The results of different concentrations of Azorhizobium caulinodans showed that the root length and plant height of'Fulza 2'seedlings increased significantly at the concentration of 1.0×108 rhizobium/mL,and the colonization of rhizobium in the root tissues of healthy'Fulza 2'seedlings was observed by fluorescence microscopy.After 3 days of treatment,rhizobium could invade and colonize the roots of'Fulza 2'seedlings.After 15 days of inoculation,high population density was developed in the local tis-sues of stems.Horizontal comparison of the three inducers'nodulation efficiency showed that the three inducers could form nodule in the roots of stem tumor mustard after 30 days,and the number of nodule increased significantly after 60 days.The nodulation efficiency of 2,4-D was the best,up to 93%,which was significantly higher than that of the other two inducers.[Conclusion]This study lays the foundation for better utili-zing the symbiotic system of rhizobia to promote the growth of stem mustard.关键词
茎瘤芥/田菁茎瘤固氮根瘤菌/诱导结瘤/促生作用Key words
Tumorous stem mustard/Azorhizobium caulinodans/Induction nodulation/Growth-promoting effect分类
农业科技引用本文复制引用
毛仲玉,常黎洁,王殿东..根瘤菌对茎瘤芥的促生作用研究[J].安徽农业科学,2024,52(12):1-6,12,7.基金项目
重庆市高校创新研究群体项目(CXQT21029). （CXQT21029）
