安徽农业科学2024，Vol.52Issue(12)：136-138,3.DOI:10.3969/j.issn.0517-6611.2024.12.029
不同种植区库尔勒香梨树腐烂病菌Cytospora pyri营养体亲和性研究
Study on Vegetative Compatibility of Cytospora pyri from Korla Fragrant Pear in Different Planting Areas
摘要
Abstract
[Objective]To use Nit mutant system to explore the genetic diversity of fragrant pear from different geographical sources in Xin-jiang.[Method]In this study,Nit mutant technology(nitrate-resistant defective mutant)was used for the first time to study the nutritional af-finity of Cytospora pyri,13 strains of Korla pear tree rot from three different growing areas in southern Xinjiang.[Result]115 Nit mutants were cultured in chlorate medium(KPS)from 13 strains,and 41 of them did not mutate.In the identification of mutant stability,33 stable Nit mu-tants and 82 unstable Nit mutants were identified.The phenotype of the resulting Nit mutants was identified as NitD 85%and NitB 15%.[Con-clusion]In this study,two Nit mutants were obtained,indicating that there may be two mutations in the nitrogen assimilation pathway of C.pyri.All isolates tested were able to utilize hypoxanthine,and the ability to use hypoxanthine ruled out the possibility that molybdenum cofactor sites were blocked.关键词
香梨树腐烂病菌/Cytospora pyri/Nit突变体Key words
Pear Valsa Canker/Cytospora pyri/Nit mutant分类
农业科技引用本文复制引用
彭莎莎,王喆,沙帅帅,张怡雯,闫成才,王兰..不同种植区库尔勒香梨树腐烂病菌Cytospora pyri营养体亲和性研究[J].安徽农业科学,2024,52(12):136-138,3.基金项目
国家自然基金-新疆联合基金重点支持项目(U1903206) （U1903206）
旱区作物逆境生物学国家重点实验室开放课题项目(CSBAA2020009) （CSBAA2020009）
自治区研究生科研与创新项目(XJ2021G293). （XJ2021G293）
