安徽农业科学2024，Vol.52Issue(12)：158-163,169,7.DOI:10.3969/j.issn.0517-6611.2024.12.034
基于斑马鱼模型对药食同源中药饮品的抗氧化和抗疲劳功效研究
Study on Anti-oxidation and Anti-fatigue Effects of Medicinal and Edible Homologous Beverage for TCM Based on Zebrafish Model
摘要
Abstract
[Objective]To analyze the chemical constituents in 2 medicinal and edible homologous beverage(Xiangfeng Yueyan Drink and Shenqi Guyuan decoction),and evaluate the anti-oxidant and anti-fatigue effects.[Method]UHPLC-TQ-Orbitrap-MS mass spectrometer and reverse phase C18 column were used to analyze the composition of the samples.The oxidative stress model established by aqueous menadione administration method was added with different gradient concentrations(25,50,100 μg/mL)of Xiangfeng Yueyan Drink dilution.Meanwhile,a normal group and a positive drug control group were set up.After treatment for 24 h,the Cell ROX was stained without light,and the fluores-cence intensity of yolk sac was calculated by fluorescence microscope.Select 5-day-old wild type zebrafish and add different gradient concen-trations(25,50,100 μg/mL)of Shenqi Guyuan decoction diluent.A normal control group and a positive drug group were set up at the same time.After treatment for 18 h,the fatigue model of zebrafish was established by aqueous solution of anhydrous sodium sulfite in the administra-tion group,and the total movement distance of zebrafish was analyzed by behavior analyzer.[Result]According to the mass spectrometry data,63 compounds were detected in Xiangfeng Yueyan Drink and 60 compounds were detected in Shenqi Guyuan Drink.Compared with the model group,the fluorescence intensity of zebrafish yolk sac significantly weakened under the intervention of Xiangfeng Yueyan Drink(P＜0.01);compared with the model control group,the total movement distance of zebrafish under the intervention of Shenqi Guyuan Drink was signifi-cantly longer(P＜0.05).[Conclusion]The 2 kinds of medicinal and edible homologous beverage have clear anti-oxidation and anti-fatigue effects respectively.关键词
斑马鱼/药食同源/液质联用/抗氧化/抗疲劳Key words
Zebrafish/Homology of medicine and food/LC-MS/Anti-oxidation/Anti-fatigue分类
轻工纺织引用本文复制引用
孙博,郑海云,王超超,刘玉洋,王梦晓,田孟尧,王慧君,赵海誉..基于斑马鱼模型对药食同源中药饮品的抗氧化和抗疲劳功效研究[J].安徽农业科学,2024,52(12):158-163,169,7.基金项目
中国中医科学院科技创新工程项目(CI2021A04012). （CI2021A04012）
