现代农业科技Issue(12)：167-173,7.DOI:10.3969/j.issn.1007-5739.2024.12.041
丹参根和叶中丹参酮与丹酚酸含量差异的分子机制研究
Molecular Mechanisms of Differences in Tanshinone and Salvianolic Acid Content in Salvia miltiorrhiza Root and Leaf
摘要
Abstract
The medicinal parts of Salvia miltiorrhiza are dry roots and stems,and leaves are not used as medicinal parts.In order to analyze the differences in drug efficacy among different tissue parts of Salvia miltiorrhiza,this study used the Illumina high-throughput sequencing platform to complete transcriptome sequencing and functional annotation of the medicinal roots and non medicinal leaves of Salvia miltiorrhiza.The differentially expressed genes in the roots and leaves were analyzed and compared,and gene depth mining was carried out.The results showed that the percentage of Q20 bases in various products was above 98.11%,and the percentage of Q30 bases was above 94.37%.There were 6 959 differentially expressed genes in roots and leaves,of which 3 265 genes were upregulated and 3 694 genes were downregulated.GO enrichment analysis showed that differentially expressed genes were mainly enriched in pathways such as light harvesting of photosynthesis,light harvesting in photosystemⅠof photosynthesis,photosynthetic electron transport in photosystemⅠand thylakoid lumen.Differential gene analysis was performed on the biosynthesis pathways of tanshinone(diterpenoid metabolism pathway map00904)and salvianolic acid(phenylpropanoid metabolism pathway map00940),and two differentially expressed PAL genes and two differentially expressed CPS genes were obtained,respectively.These differentially expressed genes may be related to the accumulation of tanshinone and salvianolic acid mainly in the roots of Salvia miltiorrhiza.The results of this study provide rich information for the exploration of functional genes involved in the biosynthesis of tanshinone and salvianolic acid,as well as the analysis of secondary metabolic pathways of pharmacological substances.关键词
丹参/转录组测序/丹参酮/丹酚酸/分子机制Key words
Salvia miltiorrhiza/transcriptome sequencing/tanshinone/salvianolic acid/molecular mechanism分类
农业科技引用本文复制引用
陆心怡,姜浩琪,李敏,李思雨,翟科峰,刘发旺..丹参根和叶中丹参酮与丹酚酸含量差异的分子机制研究[J].现代农业科技,2024,(12):167-173,7.基金项目
安徽省大学生创新创业训练计划项目(S202310379105) （S202310379105）
宿州学院博士科研启动基金项目(2022BSK033) （2022BSK033）
国家级/省级大学生创新创业训练计划项目(202310379014,202310379014) （202310379014,202310379014）
安徽省高校优秀科研创新团队项目(2022AH010080). （2022AH010080）
