中医康复2024，Vol.1Issue(8)：9-15,7.DOI:10.19787/j.issn.2097-3128.2024.08.002
乌头汤调控lncRNA MALAT1/miR-124途径抑制软骨细胞焦亡的机制研究
Mechanism of Wutou Decoction Regulating lncRNA MALAT1/miR-124 Pathway and Inhibiting Chondrocyte Pyroptosis
摘要
Abstract
Objective:To investigate whether Wutou decoction inhibits chondrocyte pyroptosis by regulating the Lnc RNA MALAT1/miR-124 path-way.Methods:LPS(1μg/mL)24h+ATP(5.5mmol/L)4h were used to induce pyroptosis in chondrocytes,and the lncRNA MALAT1 silencing cell line was constructed.Chondrocytes were randomLy divided into blank control group(Lenti-control),virus silencing group(sh-MALAT1),model con-trol group(LPS+ATP+Lenti-control),model silencing group(LPS+ATP+sh-MALAT1).The medicated serum of Wutou decoction was prepared,and the cells were randomly divided into blank group,model group(LPS+ATP)and Wutou decoction group(LPS+ATP+WTD medicated serum)for in-tervention.Real-time PCR was used to detect the changes of lncRNA MALAT1 and miR-124 genes in chondrocytes in each group.Western blot was used to detect the protein content of NLRP3,caspase-1,GSDMD,IL-1β and IL-18 in chondrocytes in each group.Results:The expression of ln-cRNA MALAT1 was increased in the nucleus of lncRNA achondrocytes induced by LPS+ATP.Compared with the blank control group,the protein contents of NLRP3,caspase-1,GSDMD,IL-1β and IL-18 in the model silencing group decreased(P<0.05),while the content of the above index pro-teins in the virus silencing group decreased compared with the model control group(P<0.05).Compared with the model group,the expression of ln-cRNA MALAT1 and the above indexes decreased(P<0.05),and the mRNA expression of miRNA-124 increased(P<0.05).Conclusion:Wutou decoc-tion can inhibit chondrocyte pyroptosis by regulating the lncRNA MALAT1/miR-124 pathway.关键词
乌头汤/lncRNA MALAT1/miR-124/细胞焦亡/软骨细胞Key words
Wutou decoction/lncRNA MALAT1/miR-124/pyroptosis/chondrocytes分类
医药卫生引用本文复制引用
林艳铭,付长龙,李宇涛,涂海水,陈悦,仲卫红..乌头汤调控lncRNA MALAT1/miR-124途径抑制软骨细胞焦亡的机制研究[J].中医康复,2024,1(8):9-15,7.基金项目
福建省卫健委科技计划项目(2021GG01010157) （2021GG01010157）
福建省自然科学基金项目(2022J01374) （2022J01374）
