Characterization of distinct microbiota associated with androgenetic alopecia patients treated and untreated with platelet-rich plasma(PRP)OA

Background:Androgenic alopecia(AGA)is the most common type of hair loss in men,and there are many studies on the treatment of hair loss by platelet-rich plasma(PRP).The human scalp contains a huge microbiome,but its role in the process of hair loss remains unclear,and the relationship between PRP and the microbiome needs further study.Therefore,the purpose of this study was to investigate the effect of PRP treat-ment on scalp microbiota composition. Methods:We performed PRP treatment on 14 patients with AGA,observed their clin-ical efficacy,and collected scalp swab samples before and after treatment.The scalp microflora of AGA patients before and after treatment was characterized by amplify-ing the V3-V4 region of the 16 s RNA gene and sequencing for bacterial identification. Results:The results showed that PRP was effective in the treatment of AGA pa-tients,and the hair growth increased significantly.The results of relative abundance analysis of microbiota showed that after treatment,g_Cutibacterium increased and g_Staphylococcus decreased,which played a stable role in scalp microbiota.In addi-tion,g_Lawsonella decreased,indicating that the scalp oil production decreased after treatment. Conclusions:The findings suggest that PRP may play a role in treating AGA through scalp microbiome rebalancing.