风湿病与关节炎2024，Vol.13Issue(6)：1-4,10,5.DOI:10.3969/j.issn.2095-4174.2024.06.001
miR-146a调控TLRs信号通路对类风湿关节炎患者外周血单个核细胞的影响
The Effect of miR-146a Regulating TLRs Signaling Pathway on Peripheral Blood Mononuclear Cells in Patients with Rheumatoid Arthritis
摘要
Abstract
Objective:To observe the effect of miR-46 regulation of TLRs signaling pathway on peripheral blood mononuclear cells in patients with active rheumatoid arthritis.Methods:Twenty patients with active rheumatoid arthritis were selected and divided into the LPS group and the LPS+miR-146a group,and peripheral blood mononuclear cells in them were isolated and cultured.At the same time,ten healthy examinees were selected as the healthy control group.MTT method was used to measure absorbance,changes in peripheral blood mononuclear cells were compared,and apoptosis of peripheral blood mononuclear cells was detected.RT-PCR was used to detect the mRNA expression of miR-146a,TLR2,and MyD88 in peripheral blood mononuclear cells.Protein immunoblotting was used to detect the protein expression of miR-146a,TLR2,and MyD88 in peripheral blood mononuclear cells.Results:The TLR2 expression rate of peripheral blood mononuclear cells in the LPS group was significantly increased compared to the healthy control group and the LPS+miR-146a group(P＜0.05);the expression of protein mRNA of miR-146a,TLR2,and MyD88 in peripheral blood mononuclear cells of the LPS group was significantly increased compared to the healthy control group and the LPS+miR-146a group(P＜0.05);the protein levels of miR-146a,TLR2,and MyD88 in peripheral blood mononuclear cells were significantly increased in the LPS group compared to the healthy control group and the LPS+miR-146a group(P＜0.05).Conclusion:High expression may inhibit the expression of TLR2 and MyD88 in peripheral blood mononuclear cells of patients with active rheumatoid arthritis by relying on the TLR2/MyD88 signaling pathway,reducing inflammatory response.关键词
类风湿关节炎/外周血单个核细胞/miR-146/Toll样受体2/髓样分化因子88Key words
rheumatoid arthritis/peripheral blood mononuclear cells/miR-146a/TLR2/MyD88引用本文复制引用
闫慧明,刘河红,张雪,张丽霞,闫师,薛一萍,孙英梅..miR-146a调控TLRs信号通路对类风湿关节炎患者外周血单个核细胞的影响[J].风湿病与关节炎,2024,13(6):1-4,10,5.基金项目
2022年度内蒙古自治区高等学校科学技术研究项目(NJZY22083) （NJZY22083）
