赣南医学院学报2024，Vol.44Issue(5)：488-494,7.DOI:10.3969/j.issn.1001-5779.2024.05.007
基于Box-Behnken响应面设计优选乌蕨总黄酮的超声提取工艺
Optimization of ultrasonic extraction process of total flavonoids from Stenoloma chusanum(L.)Ching based on Box-Behnken response surface methodology
王若乔 1袁丰华 2罗娟娟 3廖梅香 1温慧玲1
作者信息
- 1. 赣南医科大学药学院
- 2. 赣南医科大学生物技术教研室
- 3. 赣南医科大学基础医学院分子病理学中心,江西 赣州 341000
- 折叠
摘要
Abstract
Objective:To optimize the ultrasonic-assisted extraction process of total flavonoids from Stenoloma chusanum(L.)Ching using Box-Behnken response surface methodology.Methods:Single-factor experiments were conducted to explore three influencing factors:ethanol concentration,liquid-solid ratio,ultrasonic temperature.Regarding the extraction amount of total flavonoids as the evaluation index,the combination design of Box-Behnken was combined to optimize the optimal process of total flavonoids extraction.Results:The optimum process conditions for ultrasonic extraction of total flavonoids from Stenoloma chusanum were as follows:ethanol concentration 34％,liquid-solid ratio 30∶1(Ml·g-1),ultrasonic extraction temperature 72 ℃,ultrasonic time 40 min.Under these conditions,the predicted value of response surface was 46.132 mg·g-1,the measured average value was 46.052 mg·g-1,RSD=1.61％.Conclusion:The verification results show that this method is practical and reliable,and the effect is good.It can be applied to the extraction of total flavonoids from Stenoloma chusanum,which provides theoretical reference for the extraction of total flavonoids from Stenoloma chusanum.关键词
乌蕨/总黄酮/超声波/响应面优化/提取工艺Key words
Stenoloma chusanum/Total flavonoids/Ultrasonic/Response surface optimization/Extraction process分类
中医学引用本文复制引用
王若乔,袁丰华,罗娟娟,廖梅香,温慧玲..基于Box-Behnken响应面设计优选乌蕨总黄酮的超声提取工艺[J].赣南医学院学报,2024,44(5):488-494,7.
