现代农业装备2024，Vol.45Issue(3)：63-68,6.
基于衍射重建聚焦度评价的猪精子三维定位方法研究
Study on 3D Positioning Method of Porcine Sperm based on Focusing Degree Evaluation of Diffraction Reconstruction
摘要
Abstract
Sperm motility performance is an important basis for evaluating sperm motility.At present,the relatively advanced computer-assisted sperm analysis(CASA)method uses graphics processing technology to process and analyze sperm images taken by phase contrast microscope.The equipment is large and expensive,and the imaging field is small.More importantly,the motion trajectory obtained by this method is on the plane.In fact,sperm movement occurs in three dimensions.The key step to obtain 3D motion trajectory is 3D positioning.Therefore,a method to obtain 3D positioning of porcine sperm by lensless diffraction imaging is proposed in this study.In this method,based on the diffraction image reconstruction,image processing algorithm is used to obtain its plane coordinates,and focusing degree evaluation index and self-focusing algorithm are designed to obtain its height coordinates by using the principle that the reconstruction distance affects the contrast of virtual and real images.Furthermore,the 3D location of porcine sperm individual is realized.The results show that this method can achieve the accuracy of±1.4 μm and±10 μm in the horizontal and vertical directions respectively.This method is expected to provide more scientific data support for sperm quality detection and reproductive medicine research.关键词
猪精子/三维定位/衍射重建/聚焦度评价/自聚焦算法Key words
porcine sperm/three-dimensional positioning/diffraction reconstruction/focusing degree evaluation/self-focusing algorithm分类
农业科技引用本文复制引用
李国晓,姜玥彤,李晨曦,武君伟..基于衍射重建聚焦度评价的猪精子三维定位方法研究[J].现代农业装备,2024,45(3):63-68,6.基金项目
江苏省政策引导类项目——苏北科技专项(SZ-LYG202113) （SZ-LYG202113）
江苏农林职业技术学院青年扶持项目(2021kj31) （2021kj31）
江苏农林职业技术学院2023年大学生创新创业培育计划项目(202313103082y) （202313103082y）
