海南师范大学学报（自然科学版）2024，Vol.37Issue(2)：158-163,6.DOI:10.12051/j.issn.1674-4942.2024.02.004
基于二氰基异佛尔酮的荧光探针用于细胞中半胱氨酸的检测
A Fluorescent Probe Based on Isophorone Dicyanide for the Detection of Cysteine in Cells
摘要
Abstract
Cysteine(Cys)plays an indispensable role in biological systems,which can participate in a variety of physiologi-cal and pathological processes in the human body and plays an essential role in the regulation of redox homeostasis and cell activity.A large number of studies have shown that abnormal Cys concentrations are strongly associated with the occur-rence of various diseases.Therefore,it is necessary to develop a specific fluorescence probe for Cys detection.In this paper,a fluorescent probe ISO-EV-AC was designed and synthesized using acryloyl group as the recognition unit and isophorone dicyano as the fluorophore.The results indicated that probe ISO-EV-AC exhibits a high selectivity for the detection of Cys without interference from the other nanlytes.The detection limit was as low as 31 nmol/L and the response time of Cys was 5 min.The results of the cell experiments demonstrated that probe ISO-EV-AC can be used to detect Cys in cells.关键词
半胱氨酸/荧光探针/丙烯酰氯/二氰基异佛尔酮Key words
cysteine/fluorescent probe/acryloyl chloride/isophorone dicyano分类
化学化工引用本文复制引用
王志宇,沈舒婷,杨静,胡磊,王慧..基于二氰基异佛尔酮的荧光探针用于细胞中半胱氨酸的检测[J].海南师范大学学报（自然科学版）,2024,37(2):158-163,6.基金项目
安徽省自然科学基金项目(1908085QB50) （1908085QB50）
安徽省大学生创新创业教育训练计划项目(S202310368089) （S202310368089）
