多组织蜡块在免疫组织化学染色阳性对照中的应用OACSTPCD
Application of multi-tissue paraffin blocks in positive controls for immunohistochemical staining
目的:目前免疫组织化学染色阳性对照的制作方法多种多样,单组织对照制作简单,但使用时由于免疫组织化学染色指标过多,容易出错.一些多组织对照使用方便,但制作方法繁琐、难度高.本研究旨在探讨一种新型、简单的多组织蜡块的制作及其在免疫组织化学染色对照中的应用.方法:依据国际特设专家委员会对常见抗体对照组织的推荐,选取包含肝、阑尾、扁桃体、肺、胰腺组织蜡块各1个为1组,共10组,进行厚度为6 μm的连续切片,将切好的组织片依次卷曲叠在一起,形成一个长条形的…查看全部>>
Objective:Currently,there are various methods for producing positive controls for immunohistochemical staining.Single-tissue controls are simple to make,but their use can lead to errors due to the multitude of immunohistochemical staining indicators.Some multi-tissue controls are convenient to use but are complex and difficult to produce.This study aims to explore a novel and simple method for creating multi-tissue paraffin blocks and their application in im…查看全部>>
张贝;刘永桥
应城市人民医院病理科,湖北孝感 432400应城市人民医院病理科,湖北孝感 432400
免疫组织化学染色阳性对照多组织蜡块
immunohistochemical stainingpositive controlmulti-tissue paraffin block
《临床与病理杂志》 2024 (3)
390-398,9
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